Overview

  • Product name

    Lipid Peroxidation (4-HNE) Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
  • Assay type

    Competitive
  • Species reactivity

    Reacts with: Vertebrata
  • Product overview

    Lipid Peroxidation (4-HNE) Assay Kit (ab238538) is designed for the rapid detection and quantitation of  4-hydroxynonenal (4-HNE)-protein adducts.


    4-HNE is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. This Competitive ELISA kit  will allow the quantitation of 4-HNE adduct in protein samples determined by comparing its absorbance with that of a known 4-HNE-BSA standard curve.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests 32 tests
    100X Conjugate Diluent 1 x 300µl 1 x 100µl
    10X Wash Buffer 1 x 100ml 1 x 30ml
    Protein Binding Strip Well Plate 1 unit 1 unit
    Anti-HNE Antibody (1000X) 1 x 10µl 1 x 5µl
    Assay Diluent 1 x 50ml 1 x 20ml
    HNE Conjugate 1 x 50µl 1 x 20µl
    HNE-BSA Standard 1 x 250µl 1 x 100µl
    Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl 1 x 20µl
    Stop Solution 1 x 12ml 1 x 4ml
    Substrate Solution 1 x 12ml 1 x 4ml
  • Research areas

  • Relevance

    Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • 4 HNE
    • 4-Hydroxy-2-Nonenal

Images

  • The following figures demonstrate typical HNE Adduct Competitive ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.

Protocols

References

This product has been referenced in:

  • Devarajan A  et al. Maternal perinatal calorie restriction temporally regulates the hepatic autophagy and redox status in male rat. Free Radic Biol Med 130:592-600 (2019). Read more (PubMed: 30248445) »
See 1 Publication for this product

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