Key features and details
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
Product nameLipid Peroxidation Assay Kit (Cell-based)
See all Lipid Peroxidation kits
Lipid Peroxidation Assay Kit (Cell-based) (ab243377) uses a sensitive ratiometric Lipid Peroxidation Sensor that changes its fluorescence from red to green upon peroxidation by ROS in cells, this peroxidation-dependent shift enables the ratiometric measurement of lipid peroxidation. Our kit includes H2O2 as a positive control treatment to induce lipid peroxidation.
Tested applicationsSuitable for: Flow Cyt, FMmore details
PlatformFlow cytometer, Fluorescence microscope
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests 3% H2O2 1 x 100µl HHBS 1 x 50ml Ratiometric lipid peroxidation sensor R590/G525 (500X) 1 x 50µl
Our Abpromise guarantee covers the use of ab243377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|FM||Use at an assay dependent concentration.|
For H2O2 treatment, approximately 250 µM H2O2 was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid peroxidation Sensor, and stained with Hoechst 33342 during the last 10 minutes of incubation. The cells were washed 3 times with HHBS and imaged with a Keyence fluorescent microscope. With H2O2 treatment, a clear shift of fluorescence signal of red to green was observed.
For H2O2 treatment, approximately 250 µM H2O2 was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid Peroxidation Sensor, and analyzed with a flow cytometer through FITC (488/530 nm) and PE (488/572 nm) channels. The data are represented as the ratios of red (PE)/green (FITC) fluorescence intensities. The ratio of red/green decreases in H2O2 treated cells indicating the presence of H2O2-induced lipid peroxidation.
ab243377 has not yet been referenced specifically in any publications.