Key features and details
- Rabbit polyclonal to Lipin 1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Lipin 1 antibody
See all Lipin 1 primary antibodies
DescriptionRabbit polyclonal to Lipin 1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Lipin 1 (C terminal). The exact sequence is proprietary. 18 amino acids.
Database link: Q14693
- K562 cell lysate. Human small intestine tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab181389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 99 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionPlays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
Tissue specificityAbundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
Involvement in diseaseDefects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
Sequence similaritiesBelongs to the lipin family.
DomainContains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
modificationsPhosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
Cellular localizationNucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
- Information by UniProt
- EC=220.127.116.11 antibody
- KIAA0188 antibody
- Lipin-1 antibody
Anti-Lipin 1 antibody (ab181389) at 1 µg/ml + K562 whole cell lysate at 15 µg
Predicted band size: 99 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of Human small intestine tissue, staining Lipin 1 with ab181389 at 5 μg/ml.
Immunfluorescence analysis of Human small intestine tissue, staining Lipin 1 with ab181389 at 20 μg/ml.
ab181389 has been referenced in 3 publications.
- Zhao Y et al. Gain-of-Function Mutations of SLC16A11 Contribute to the Pathogenesis of Type 2 Diabetes. Cell Rep 26:884-892.e4 (2019). PubMed: 30673611
- Peng Q et al. Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes. Aging (Albany NY) 11:11520-11540 (2019). PubMed: 31831718
- Cheng XY et al. miR-203 Inhibits Alcohol-Induced Hepatic Steatosis by Targeting Lipin1. Front Pharmacol 9:275 (2018). PubMed: 29670525