Key features and details
- Rabbit polyclonal to Lipin 1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Lipin 1 antibody
See all Lipin 1 primary antibodies
DescriptionRabbit polyclonal to Lipin 1
Tested Applications & Species
Application Species IPHuman
Synthetic peptide within Human Lipin 1 aa 225-275. The exact sequence is proprietary.
Database link: Q14693
- IP: Jurkat whole cell lysate.
ab241134 has not performed satisfactorily when used for WB of Lipin 1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Lipin 1.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
Purification notesAntibody was affinity purified using an epitope specific to Lipin 1 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use at 2-10 µg/mg of lysate.
Use at 2-10 µg/mg of lysate.
FunctionPlays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
Tissue specificityAbundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
Involvement in diseaseDefects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
Sequence similaritiesBelongs to the lipin family.
DomainContains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
modificationsPhosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
Cellular localizationNucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
- Information by UniProt
- EC=18.104.22.168 antibody
- KIAA0188 antibody
- Lipin-1 antibody
Lipin 1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241134 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241134 at 1 µg/ml.
Lane 1: ab241134 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241134 has not yet been referenced specifically in any publications.