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    lipin-1-antibody-ab70138.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Anti-Lipin 1 antibody (ab70138)

  • Datasheet
Reviews (4) Submit a question References (5)

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Western blot - Anti-Lipin 1 antibody (ab70138)

    Key features and details

    • Rabbit polyclonal to Lipin 1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Lipin 1 antibody
      See all Lipin 1 primary antibodies
    • Description

      Rabbit polyclonal to Lipin 1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee
    • Immunogen

      Synthetic peptide corresponding to Human Lipin 1 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab86631)

    • Positive control

      • This antibody gave a positive signal in the following Mouse Tissue Lysates: Liver; Pancreas, Kidney.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cell Biology
      • Cell Cycle
      • Cell differentiation
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Types of disease
      • Diabetes
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab70138 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

    Target

    • Function

      Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
    • Tissue specificity

      Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
    • Involvement in disease

      Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
    • Sequence similarities

      Belongs to the lipin family.
    • Domain

      Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
      Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
    • Post-translational
      modifications

      Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
      Sumoylated.
    • Cellular localization

      Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession Q14693 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23175 Human
      • Entrez Gene: 14245 Mouse
      • Entrez Gene: 313977 Rat
      • Omim: 605518 Human
      • SwissProt: Q14693 Human
      • SwissProt: Q91ZP3 Mouse
      • Unigene: 467740 Human
      • Unigene: 153625 Mouse
      • Alternative names

        • EC=3.1.3.4 antibody
        • KIAA0188 antibody
        • Lipin-1 antibody
        • Lpin1 antibody
        • LPIN1_HUMAN antibody
        • PAP1 antibody
        • Phosphatidate phosphatase LPIN1 antibody
        see all

      Images

      • Western blot - Anti-Lipin 1 antibody (ab70138)
        Western blot - Anti-Lipin 1 antibody (ab70138)
        All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml

        Lane 1 : Liver (Mouse) Tissue Lysate
        Lane 2 : Pancreas (Mouse) Tissue Lysate
        Lane 3 : Kidney (Mouse) Tissue Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 99 kDa
        Observed band size: 110 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (5)

      Publishing research using ab70138? Please let us know so that we can cite the reference in this datasheet.

      ab70138 has been referenced in 5 publications.

      • Guo Z  et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
      • Lin YC  et al. Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30934807
      • Lizardo K  et al. Diet Alters Serum Metabolomic Profiling in the Mouse Model of Chronic Chagas Cardiomyopathy. Dis Markers 2019:4956016 (2019). PubMed: 31949545
      • Zhang W  et al. Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury. Sci Rep 8:408 (2018). WB ; Mouse . PubMed: 29323242
      • Cai K  et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). WB . PubMed: 24369117

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Lipin 1 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Airway epithelial cells)
      Specification
      Airway epithelial cells
      Fixative
      Acetone
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
      Read More

      Prof. Marc Chanson

      Verified customer

      Submitted Sep 07 2012

      Western blot abreview for Anti-Lipin 1 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (white adipose tissue)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      20 µg
      Specification
      white adipose tissue
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 28 2018

      Western blot abreview for Anti-Lipin 1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Heart)
      Loading amount
      30 µg
      Specification
      Heart
      Gel Running Conditions
      Reduced Denaturing (8%)
      Blocking step
      BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 07 2012

      Western blot abreview for Anti-Lipin 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (mouse heart)
      Loading amount
      50 µg
      Specification
      mouse heart
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 23 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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