- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Lipin 1 antibody
See all Lipin 1 primary antibodies -
DescriptionRabbit polyclonal to Lipin 1
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human Lipin 1.
(Peptide available as ab86631.)
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Positive control
- This antibody gave a positive signal in the following Mouse Tissue Lysates: Liver; Pancreas, Kidney.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab70138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). |
Target
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FunctionPlays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
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Tissue specificityAbundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
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Involvement in diseaseDefects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
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Sequence similaritiesBelongs to the lipin family.
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DomainContains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity. -
Post-translational
modificationsPhosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
Sumoylated. -
Cellular localizationNucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
- Information by UniProt
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Database links
- Entrez Gene: 23175 Human
- Entrez Gene: 14245 Mouse
- Entrez Gene: 313977 Rat
- Omim: 605518 Human
- SwissProt: Q14693 Human
- SwissProt: Q91ZP3 Mouse
- Unigene: 467740 Human
- Unigene: 153625 Mouse
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Alternative names
- EC=3.1.3.4 antibody
- KIAA0188 antibody
- Lipin-1 antibody
see all
Images
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Western blot - Anti-Lipin 1 antibody (ab70138)All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Zhang W et al. Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury. Sci Rep 8:408 (2018). WB ; Mouse . Read more (PubMed: 29323242) »
- Cai K et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). WB . Read more (PubMed: 24369117) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"