Anti-Lipin 1 antibody (ab70138)
Key features and details
- Rabbit polyclonal to Lipin 1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Lipin 1 antibody
See all Lipin 1 primary antibodies -
Description
Rabbit polyclonal to Lipin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Human Lipin 1 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab86631) -
Positive control
- This antibody gave a positive signal in the following Mouse Tissue Lysates: Liver; Pancreas, Kidney.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab70138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). |
Target
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Function
Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. -
Tissue specificity
Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract. -
Involvement in disease
Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years. -
Sequence similarities
Belongs to the lipin family. -
Domain
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity. -
Post-translational
modificationsPhosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 23175 Human
- Entrez Gene: 14245 Mouse
- Entrez Gene: 313977 Rat
- Omim: 605518 Human
- SwissProt: Q14693 Human
- SwissProt: Q91ZP3 Mouse
- Unigene: 467740 Human
- Unigene: 153625 Mouse
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Alternative names
- EC=3.1.3.4 antibody
- KIAA0188 antibody
- Lipin-1 antibody
see all
Images
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Western blot - Anti-Lipin 1 antibody (ab70138)All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (5)
ab70138 has been referenced in 5 publications.
- Guo Z et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
- Lin YC et al. Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30934807
- Lizardo K et al. Diet Alters Serum Metabolomic Profiling in the Mouse Model of Chronic Chagas Cardiomyopathy. Dis Markers 2019:4956016 (2019). PubMed: 31949545
- Zhang W et al. Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury. Sci Rep 8:408 (2018). WB ; Mouse . PubMed: 29323242
- Cai K et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). WB . PubMed: 24369117
