Overview

  • Product name

    Anti-Lipoamide Dehydrogenase antibody
    See all Lipoamide Dehydrogenase primary antibodies
  • Description

    Rabbit polyclonal to Lipoamide Dehydrogenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Lipoamide Dehydrogenase aa 1-509.
    Database link: P09622

  • Positive control

    • MCF7, BT474, K562, 293T cell extracts; mouse kidney, skeletal muscle and liver extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab186827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 54 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
  • Involvement in disease

    Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
    Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
  • Sequence similarities

    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Post-translational
    modifications

    Tyrosine phosphorylated.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dehydrogenase complex, E3 component antibody
    • Diaphorase antibody
    • Dihydrolipoamide dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase mitochondrial antibody
    • dld antibody
    • DLDD antibody
    • DLDH antibody
    • DLDH_HUMAN antibody
    • E3 antibody
    • E3 branched chain aplha-keto acid antibody
    • E3 component of pyruvate dehydrogenase antibody
    • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
    • GCSL antibody
    • Glycine cleavage system L protein antibody
    • Glycine cleavage system protein L antibody
    • LAD antibody
    • lipoamide dehydrogenase antibody
    • Lipoamide reductase antibody
    • Lipoyl dehydrogenase antibody
    • mitochondrial antibody
    • OTTHUMP00000206744 antibody
    • OTTHUMP00000206746 antibody
    • OTTHUMP00000206748 antibody
    • OTTHUMP00000206749 antibody
    • PHE 3 antibody
    • PHE3 antibody
    • Pyruvate dehydrogenase component E3 antibody
    see all

Images

  • All lanes : Anti-Lipoamide Dehydrogenase antibody (ab186827) at 1/500 dilution

    Lane 1 : MCF7 cell extracts
    Lane 2 : BT474 cell extracts
    Lane 3 : K562 cell extracts
    Lane 4 : 293T cell extracts
    Lane 5 : Mouse kidney cell extracts
    Lane 6 : Mouse skeletal muscle cell extracts
    Lane 7 : Mouse liver cell extracts

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 400x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186827. Blue DAPI for nuclear staining.

References

ab186827 has not yet been referenced specifically in any publications.

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