Key features and details
- Rabbit polyclonal to Lipoamide Dehydrogenase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Lipoamide Dehydrogenase antibody
See all Lipoamide Dehydrogenase primary antibodies
DescriptionRabbit polyclonal to Lipoamide Dehydrogenase
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Lipoamide Dehydrogenase aa 1-509.
Database link: P09622
- MCF7, BT474, K562, 293T cell extracts; mouse kidney, skeletal muscle and liver extracts.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 54 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionLipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Involvement in diseaseNote=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Dehydrogenase complex, E3 component antibody
- Diaphorase antibody
- Dihydrolipoamide dehydrogenase antibody
All lanes : Anti-Lipoamide Dehydrogenase antibody (ab186827) at 1/500 dilution
Lane 1 : MCF7 cell extracts
Lane 2 : BT474 cell extracts
Lane 3 : K562 cell extracts
Lane 4 : 293T cell extracts
Lane 5 : Mouse kidney cell extracts
Lane 6 : Mouse skeletal muscle cell extracts
Lane 7 : Mouse liver cell extracts
Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Lipoamide Dehydrogenase with ab186827 at 1/200. Magnification: 200x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186827. Blue DAPI for nuclear staining.
ab186827 has not yet been referenced specifically in any publications.