Key features and details
- Rabbit polyclonal to Lipoamide Dehydrogenase - C-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Lipoamide Dehydrogenase antibody - C-terminal
See all Lipoamide Dehydrogenase primary antibodies
DescriptionRabbit polyclonal to Lipoamide Dehydrogenase - C-terminal
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Dog, Orangutan
Synthetic peptide within Human Lipoamide Dehydrogenase aa 459-509 (C terminal). The exact sequence is proprietary. (NP_000099.2).
Database link: P09622
- IHC-P: Human prostate carcinoma and ovarian carcinoma tissues. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226202 was affinity purified using an epitope specific to Lipoamide Dehydrogenase immobilized on solid support.
Our Abpromise guarantee covers the use of ab226202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionLipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Involvement in diseaseNote=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Dehydrogenase complex, E3 component antibody
- Diaphorase antibody
- Dihydrolipoamide dehydrogenase antibody
All lanes : Anti-Lipoamide Dehydrogenase antibody - C-terminal (ab226202) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 1 second
Lysates prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Lipoamide Dehydrogenase with ab226202 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
Lipoamide Dehydrogenase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226202 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226202 at 0.4 µg/ml.
Lane 1: ab226202 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Lipoamide Dehydrogenase with ab226202 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226202 has not yet been referenced specifically in any publications.