Recombinant
RabMAb

Recombinant Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)

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Overview

  • Product name

    Anti-Lipoamide Dehydrogenase antibody [EPR6635]
    See all Lipoamide Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human Lipoamide Dehydrogenase (UniProt ID: P09622).

  • Positive control

    • Jurkat, HeLa, 293T, MCF7, and Caco-2 cell lysates, Human kidney and Human colon tissues

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

    • Involvement in disease

      Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
      Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.

    • Sequence similarities

      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

    • Post-translational
      modifications

      Tyrosine phosphorylated.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Dehydrogenase complex, E3 component antibody
      • Diaphorase antibody
      • Dihydrolipoamide dehydrogenase antibody
      • Dihydrolipoyl dehydrogenase antibody
      • Dihydrolipoyl dehydrogenase mitochondrial antibody
      • dld antibody
      • DLDD antibody
      • DLDH antibody
      • DLDH_HUMAN antibody
      • E3 antibody
      • E3 branched chain aplha-keto acid antibody
      • E3 component of pyruvate dehydrogenase antibody
      • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
      • GCSL antibody
      • Glycine cleavage system L protein antibody
      • Glycine cleavage system protein L antibody
      • LAD antibody
      • lipoamide dehydrogenase antibody
      • Lipoamide reductase antibody
      • Lipoyl dehydrogenase antibody
      • mitochondrial antibody
      • OTTHUMP00000206744 antibody
      • OTTHUMP00000206746 antibody
      • OTTHUMP00000206748 antibody
      • OTTHUMP00000206749 antibody
      • PHE 3 antibody
      • PHE3 antibody
      • Pyruvate dehydrogenase component E3 antibody
      see all

    Images

    • Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
      Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
      All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : MCF7 cell lysate
      Lane 5 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 56 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Other - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
      Other - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Ferriero R  et al. Phenylbutyrate increases pyruvate dehydrogenase complex activity in cells harboring a variety of defects. Ann Clin Transl Neurol 1:462-70 (2014). Read more (PubMed: 25356417) »
    • Roselló-Lletí E  et al. Heart mitochondrial proteome study elucidates changes in cardiac energy metabolism and antioxidant PRDX3 in human dilated cardiomyopathy. PLoS One 9:e112971 (2014). WB ; Human . Read more (PubMed: 25397948) »
    See all 2 Publications for this product

    Publishing research using ab133551? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-Lipoamide Dehydrogenase antibody [EPR6635]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - other (Striatal cell line, pheochromocytoma cell line)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gradient gel. Laemmli buffer with 10% β-Mercaptoethanol. Samples were denatured at 55C for 10 min. Immobilon-FL transfer membranes. Overnight transfer at +4C.)
    Loading amount
    20 µg
    Specification
    Striatal cell line, pheochromocytoma cell line
    Blocking step
    SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 04 2018

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