Recombinant Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
![Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)](https://www.abcam.com/ps/products/133/ab133551/Images/ab133551-389435-anti-lipoamide-dehydrogenase-antibody-epr6635-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Lipoamide Dehydrogenase antibody [EPR6635]
See all Lipoamide Dehydrogenase primary antibodies -
Description
Rabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in Human Lipoamide Dehydrogenase (UniProt ID: P09622).
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Positive control
- WB: Jurkat, HeLa, 293T, MCF7, and Caco-2 cell lysates. IHC-P: Human kidney and Human colon tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.52 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6635 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. -
Involvement in disease
Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. -
Sequence similarities
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. -
Post-translational
modificationsTyrosine phosphorylated. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 1738 Human
- Entrez Gene: 13382 Mouse
- Entrez Gene: 298942 Rat
- Omim: 238331 Human
- SwissProt: P09622 Human
- SwissProt: O08749 Mouse
- SwissProt: Q6P6R2 Rat
- Unigene: 131711 Human
see all -
Alternative names
- Dehydrogenase complex, E3 component antibody
- Diaphorase antibody
- Dihydrolipoamide dehydrogenase antibody
see all
Images
-
Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : DLD knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab133551 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133551 Anti-Lipoamide Dehydrogenase antibody [EPR6635] was shown to specifically react with Lipoamide Dehydrogenase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265535 (knockout cell lysate ab257922) was used. Wild-type and Lipoamide Dehydrogenase knockout samples were subjected to SDS-PAGE. ab133551 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)](https://www.abcam.com/ps/products/133/ab133551/Images/ab133551-147290-anti-lipoamide-dehydrogenase-antibody-epr6635-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)](https://www.abcam.com/ps/products/133/ab133551/Images/ab133551-147292-anti-lipoamide-dehydrogenase-antibody-epr6635-western-blot.jpg)
Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)](https://www.abcam.com/ps/products/133/ab133551/Images/ab133551-147291-anti-lipoamide-dehydrogenase-antibody-epr6635-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)](https://www.abcam.com/ps/products/133/ab133551/Images/ab133551-147289-anti-lipoamide-dehydrogenase-antibody-epr6635.jpg)
OI-RD Scanning - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551) -
![Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)](https://www.abcam.com/ps/products/133/ab133551/Images/ab133551-6-benefits-of-recombinant-antibodies.png)
Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
Protocols
Datasheets and documents
References (5)
ab133551 has been referenced in 5 publications.
- Dobolyi A et al. Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation. Brain Struct Funct 225:639-667 (2020). PubMed: 31982949
- Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233
- Bugarski M et al. Multiphoton imaging reveals axial differences in metabolic autofluorescence signals along the kidney proximal tubule. Am J Physiol Renal Physiol 315:F1613-F1625 (2018). PubMed: 30132348
- Ferriero R et al. Phenylbutyrate increases pyruvate dehydrogenase complex activity in cells harboring a variety of defects. Ann Clin Transl Neurol 1:462-70 (2014). PubMed: 25356417
- Roselló-Lletí E et al. Heart mitochondrial proteome study elucidates changes in cardiac energy metabolism and antioxidant PRDX3 in human dilated cardiomyopathy. PLoS One 9:e112971 (2014). WB ; Human . PubMed: 25397948
