Product nameAnti-Lipoamide Dehydrogenase antibody [EPR6635]
See all Lipoamide Dehydrogenase primary antibodies
DescriptionRabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Lipoamide Dehydrogenase (UniProt ID: P09622).
- Jurkat, HeLa, 293T, MCF7, and Caco-2 cell lysates, Human kidney and Human colon tissues
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.52 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
FunctionLipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Involvement in diseaseNote=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Dehydrogenase complex, E3 component antibody
- Diaphorase antibody
- Dihydrolipoamide dehydrogenase antibody
All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Ferriero R et al. Phenylbutyrate increases pyruvate dehydrogenase complex activity in cells harboring a variety of defects. Ann Clin Transl Neurol 1:462-70 (2014). Read more (PubMed: 25356417) »
- Roselló-Lletí E et al. Heart mitochondrial proteome study elucidates changes in cardiac energy metabolism and antioxidant PRDX3 in human dilated cardiomyopathy. PLoS One 9:e112971 (2014). WB ; Human . Read more (PubMed: 25397948) »