Recombinant
RabMAb

Recombinant Anti-Lipoamide Dehydrogenase antibody [EPR6635] (Alexa Fluor® 488) (ab206723)

Overview

  • Product name

    Anti-Lipoamide Dehydrogenase antibody [EPR6635] (Alexa Fluor® 488)
    See all Lipoamide Dehydrogenase primary antibodies
  • Description

    Rabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Lipoamide Dehydrogenase aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P09622

  • Positive control

    • ICC/IF: MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
  • Involvement in disease

    Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
    Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
  • Sequence similarities

    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Post-translational
    modifications

    Tyrosine phosphorylated.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dehydrogenase complex, E3 component antibody
    • Diaphorase antibody
    • Dihydrolipoamide dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase mitochondrial antibody
    • dld antibody
    • DLDD antibody
    • DLDH antibody
    • DLDH_HUMAN antibody
    • E3 antibody
    • E3 branched chain aplha-keto acid antibody
    • E3 component of pyruvate dehydrogenase antibody
    • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
    • GCSL antibody
    • Glycine cleavage system L protein antibody
    • Glycine cleavage system protein L antibody
    • LAD antibody
    • lipoamide dehydrogenase antibody
    • Lipoamide reductase antibody
    • Lipoyl dehydrogenase antibody
    • mitochondrial antibody
    • OTTHUMP00000206744 antibody
    • OTTHUMP00000206746 antibody
    • OTTHUMP00000206748 antibody
    • OTTHUMP00000206749 antibody
    • PHE 3 antibody
    • PHE3 antibody
    • Pyruvate dehydrogenase component E3 antibody
    see all

Images

  • ab206723 staining Lipoamide Dehydrogenase in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206723 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

References

ab206723 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab206723.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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