Recombinant
RabMAb

Recombinant Anti-Lipoamide Dehydrogenase antibody [EPR6635] - BSA and Azide free (ab240067)

Overview

  • Product name

    Anti-Lipoamide Dehydrogenase antibody [EPR6635] - BSA and Azide free
    See all Lipoamide Dehydrogenase primary antibodies
  • Description

    Rabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Lipoamide Dehydrogenase aa 450-550. The exact sequence is proprietary.

  • General notes

    Ab240067 is the carrier-free version of ab133551. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240067 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
    • Involvement in disease

      Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
      Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
    • Sequence similarities

      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    • Post-translational
      modifications

      Tyrosine phosphorylated.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dehydrogenase complex, E3 component antibody
      • Diaphorase antibody
      • Dihydrolipoamide dehydrogenase antibody
      • Dihydrolipoyl dehydrogenase antibody
      • Dihydrolipoyl dehydrogenase mitochondrial antibody
      • dld antibody
      • DLDD antibody
      • DLDH antibody
      • DLDH_HUMAN antibody
      • E3 antibody
      • E3 branched chain aplha-keto acid antibody
      • E3 component of pyruvate dehydrogenase antibody
      • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
      • GCSL antibody
      • Glycine cleavage system L protein antibody
      • Glycine cleavage system protein L antibody
      • LAD antibody
      • lipoamide dehydrogenase antibody
      • Lipoamide reductase antibody
      • Lipoyl dehydrogenase antibody
      • mitochondrial antibody
      • OTTHUMP00000206744 antibody
      • OTTHUMP00000206746 antibody
      • OTTHUMP00000206748 antibody
      • OTTHUMP00000206749 antibody
      • PHE 3 antibody
      • PHE3 antibody
      • Pyruvate dehydrogenase component E3 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133551).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133551).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133551).

    References

    ab240067 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240067.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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