Anti-Lipoate-protein ligase A antibody (ab225683)

Overview

  • Product name

    Anti-Lipoate-protein ligase A antibody
  • Description

    Rabbit polyclonal to Lipoate-protein ligase A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity


    Predicted to work with: Escherichia coli
  • Immunogen

    Recombinant full length protein corresponding to Escherichia coli Lipoate-protein ligase A aa 1-338.
    Sequence:

    MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQ NPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTI STSIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHH GTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQV CEAITEAFFAHYGERVEAEIISPNKTPDLPNFAETFARQSSWEWNFGQAP AFSHLLDERFTWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQG CLYRADMLQQECEALLVDFPEQEKELRELSAWMAGAVR


    Database link: C4ZT68

  • Positive control

    • WB: Recombinant Escherichia coli Lipoate-protein ligase A protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab225683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • Relevance

    Function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes.
  • Database links

    Images

    • All lanes : Anti-Lipoate-protein ligase A antibody (ab225683) at 1/500 dilution

      Lane 1 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.08 µg
      Lane 2 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.04 µg
      Lane 3 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.02 µg
      Lane 4 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.01 µg

      Secondary
      All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa

    References

    ab225683 has not yet been referenced specifically in any publications.

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