Key features and details
- Rabbit polyclonal to Lipoate-protein ligase A
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-Lipoate-protein ligase A antibody
DescriptionRabbit polyclonal to Lipoate-protein ligase A
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Escherichia coli
Recombinant full length protein corresponding to Escherichia coli Lipoate-protein ligase A aa 1-338.
MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQ NPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTI STSIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHH GTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQV CEAITEAFFAHYGERVEAEIISPNKTPDLPNFAETFARQSSWEWNFGQAP AFSHLLDERFTWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQG CLYRADMLQQECEALLVDFPEQEKELRELSAWMAGAVR
Database link: C4ZT68
- WB: Recombinant Escherichia coli Lipoate-protein ligase A protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab225683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
RelevanceFunction: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes.
- SwissProt: C4ZT68 Escherichia coli
All lanes : Anti-Lipoate-protein ligase A antibody (ab225683) at 1/500 dilution
Lane 1 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.08 µg
Lane 2 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.04 µg
Lane 3 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.02 µg
Lane 4 : Recombinant Escherichia coli Lipoate-protein ligase A protein at 0.01 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225683 has not yet been referenced specifically in any publications.