Anti-Lipocalin-2 / NGAL antibody (ab41105)
Key features and details
- Rabbit polyclonal to Lipocalin-2 / NGAL
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Lipocalin-2 / NGAL antibody
See all Lipocalin-2 / NGAL primary antibodies -
Description
Rabbit polyclonal to Lipocalin-2 / NGAL -
Host species
Rabbit -
Specificity
A customer has submitted a positive Abreview for rat samples in IHC. However, customers have experienced problems with rat samples and this is likely due to the low homology between the immunogen and the rat protein (30%). Some batches of this antibody may work in rat, but this is not batch tested. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Human Lipocalin-2 / NGAL protein (ab95007) can be used as a positive control in WB. ab41105 gave a positive result in Human Colon Tissue Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Database links
- Entrez Gene: 3934 Human
- Omim: 600181 Human
- SwissProt: P80188 Human
- Unigene: 204238 Human
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Alternative names
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
Images
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Western blot - Anti-Lipocalin-2 / NGAL antibody (ab41105)Anti-Lipocalin-2 / NGAL antibody (ab41105) at 1 µg/ml + Human colon tissue lysate - total protein (ab30051) at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Lipocalin-2 / NGAL antibody (ab41105)ICC/IF image of ab41105 stained human Hek293 cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41105, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab41105 has been referenced in 15 publications.
- De La Chesnaye E et al. The gonadal expression pattern of lipocalin-2 and 24p3 receptor is modified in the gonads of the offspring of obese rats. Mol Med Rep 22:1409-1419 (2020). PubMed: 32627017
- De La Chesnaye E et al. Expression profiling of lipocalin-2 and 24p3 receptor in murine gonads at different developmental stages. Exp Ther Med 16:213-221 (2018). PubMed: 29896242
- Ferretti E et al. Impaired antimicrobial response and mucosal protection induced by ibuprofen in the immature human intestine. Pediatr Res 84:813-820 (2018). PubMed: 30341414
- Cortés-Araya Y et al. Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue. Stem Cells Dev 27:1518-1525 (2018). PubMed: 30044182
- Thorsvik S et al. Expression of neutrophil gelatinase-associated lipocalin (NGAL) in the gut in Crohn's disease. Cell Tissue Res 374:339-348 (2018). PubMed: 29869714
