Anti-Lipocalin-2 / NGAL antibody (ab63929)
Key features and details
- Rabbit polyclonal to Lipocalin-2 / NGAL
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Lipocalin-2 / NGAL antibody
See all Lipocalin-2 / NGAL primary antibodies -
Description
Rabbit polyclonal to Lipocalin-2 / NGAL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse Lipocalin-2/ NGAL. Residues surrounding amino acid 40.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab63929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). | |
| ICC | Use at an assay dependent concentration. |
Target
-
Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
-
Database links
- Entrez Gene: 3934 Human
- Entrez Gene: 16819 Mouse
- Entrez Gene: 170496 Rat
- Omim: 600181 Human
- SwissProt: P80188 Human
- SwissProt: P11672 Mouse
- SwissProt: P30152 Rat
- Unigene: 204238 Human
see all -
Alternative names
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
Images
-
Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)Verified CustomerAnti-Lipocalin-2 / NGAL antibody (ab63929) at 1/1000 dilution (12 hours at 4degC) + Rat kidney whole tissue lysate at 10 µg
Secondary
Goat anti-mouse IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 23 kDa
Exposure time: 10 secondsBlocking: 5% milk for 1 hour at 20°C.
-
Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Lipocalin-2 / NGAL using ab63929 (red) in ICC/IF.
-
Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1.5 µg/ml
Lane 1 : Uninduced Jurkat cell lysate
Lane 2 : Induced Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 70 kDa (possible non-specific binding) -
Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)Verified CustomerAll lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 2 µg/ml (12 hours at 4deg)
Lanes 1 & 4 : Healthy mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)
Lanes 2-3 : Acute kidney injury mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Donkey anti rabbit HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 1 secondDiluent: TBS - 0.1 % Tween- 5% milk
-
Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)This image is courtesy of an Abreview submitted by Mahesh Shivananjappaab63929 staining Lipocalin-2 / NGAL in the RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclona l(1/1000) was used as the secondary antibody.
-
Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 4 µg/ml
Lane 1 : Recombinant mouse Lipocalin-2/ NGAL protein, 2 ng
Lane 2 : Recombinant mouse Lipocalin-2/ NGAL protein, 10 ng
Lane 3 : Recombinant mouse Lipocalin-2/ NGAL protein, 50 ng
Predicted band size: 23 kDa
Protocols
References (42)
ab63929 has been referenced in 42 publications.
- Lu C et al. Akebia Saponin D ameliorated kidney injury and exerted anti-inflammatory and anti-apoptotic effects in diabetic nephropathy by activation of NRF2/HO-1 and inhibition of NF-KB pathway. Int Immunopharmacol 84:106467 (2020). PubMed: 32344355
- Cheng J et al. MicroRNA-761 suppresses remodeling of nasal mucosa and epithelial-mesenchymal transition in mice with chronic rhinosinusitis through LCN2. Stem Cell Res Ther 11:151 (2020). PubMed: 32272958
- Chow L et al. Antibacterial activity of human mesenchymal stem cells mediated directly by constitutively secreted factors and indirectly by activation of innate immune effector cells. Stem Cells Transl Med 9:235-249 (2020). PubMed: 31702119
- Huang T et al. Stroke Exacerbates Cancer Progression by Upregulating LCN2 in PMN-MDSC. Front Immunol 11:299 (2020). PubMed: 32153594
- Olinger E et al. Hepsin-mediated Processing of Uromodulin is Crucial for Salt-sensitivity and Thick Ascending Limb Homeostasis. Sci Rep 9:12287 (2019). PubMed: 31444371
