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    lipocalin-2--ngal-antibody-ab63929.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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Anti-Lipocalin-2 / NGAL antibody (ab63929)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (42)

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Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
  • Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)
  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
  • Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)
  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)

Key features and details

  • Rabbit polyclonal to Lipocalin-2 / NGAL
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Lipocalin-2 / NGAL antibody
    See all Lipocalin-2 / NGAL primary antibodies
  • Description

    Rabbit polyclonal to Lipocalin-2 / NGAL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Lipocalin-2/ NGAL. Residues surrounding amino acid 40.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Binding proteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Mouse Lipocalin-2 / NGAL protein (ab95008)

Applications

Our Abpromise guarantee covers the use of ab63929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC Use at an assay dependent concentration.

Target

  • Function

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity

    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Target information above from: UniProt accession P80188 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3934 Human
    • Entrez Gene: 16819 Mouse
    • Entrez Gene: 170496 Rat
    • Omim: 600181 Human
    • SwissProt: P80188 Human
    • SwissProt: P11672 Mouse
    • SwissProt: P30152 Rat
    • Unigene: 204238 Human
    • Unigene: 9537 Mouse
    • Unigene: 11303 Rat
    see all
  • Alternative names

    • 24p3 antibody
    • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
    • Alpha 2 microglobulin related protein antibody
    • HGNC:6526 antibody
    • HNL antibody
    • Lcn 2 antibody
    • Lcn2 antibody
    • Lipocalin-2 antibody
    • Migration stimulating factor inhibitor antibody
    • MSFI antibody
    • Neutrophil gelatinase associated lipocalin antibody
    • Neutrophil gelatinase associated lipocalin precursor antibody
    • Neutrophil gelatinase-associated lipocalin antibody
    • NGAL antibody
    • NGAL_HUMAN antibody
    • Oncogene 24p3 antibody
    • Oncogenic lipocalin 24p3 antibody
    • p25 antibody
    • Siderocalin antibody
    • siderocalin LCN2 antibody
    • SV40 induced 24P3 protein antibody
    • Uterocalin antibody
    see all

Images

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)Verified Customer
    Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1/1000 dilution (12 hours at 4degC) + Rat kidney whole tissue lysate at 10 µg

    Secondary
    Goat anti-mouse IgG-HRP at 1/10000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 10 seconds


    Blocking: 5% milk for 1 hour at 20°C.

  • Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)

    NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Lipocalin-2 / NGAL using ab63929 (red) in ICC/IF.

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1.5 µg/ml

    Lane 1 : Uninduced Jurkat cell lysate
    Lane 2 : Induced Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa
    Additional bands at: 70 kDa (possible non-specific binding)

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)Verified Customer
    All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 2 µg/ml (12 hours at 4deg)

    Lanes 1 & 4 : Healthy mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)
    Lanes 2-3 : Acute kidney injury mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Donkey anti rabbit HRP at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 1 second


    Diluent: TBS - 0.1 % Tween- 5% milk

  • Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)This image is courtesy of an Abreview submitted by Mahesh Shivananjappa

    ab63929 staining Lipocalin-2 / NGAL in the RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclona l(1/1000) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 4 µg/ml

    Lane 1 : Recombinant mouse Lipocalin-2/ NGAL protein, 2 ng
    Lane 2 : Recombinant mouse Lipocalin-2/ NGAL protein, 10 ng
    Lane 3 : Recombinant mouse Lipocalin-2/ NGAL protein, 50 ng

    Predicted band size: 23 kDa

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (42)

    Publishing research using ab63929? Please let us know so that we can cite the reference in this datasheet.

    ab63929 has been referenced in 42 publications.

    • Lu C  et al. Akebia Saponin D ameliorated kidney injury and exerted anti-inflammatory and anti-apoptotic effects in diabetic nephropathy by activation of NRF2/HO-1 and inhibition of NF-KB pathway. Int Immunopharmacol 84:106467 (2020). PubMed: 32344355
    • Cheng J  et al. MicroRNA-761 suppresses remodeling of nasal mucosa and epithelial-mesenchymal transition in mice with chronic rhinosinusitis through LCN2. Stem Cell Res Ther 11:151 (2020). PubMed: 32272958
    • Chow L  et al. Antibacterial activity of human mesenchymal stem cells mediated directly by constitutively secreted factors and indirectly by activation of innate immune effector cells. Stem Cells Transl Med 9:235-249 (2020). PubMed: 31702119
    • Huang T  et al. Stroke Exacerbates Cancer Progression by Upregulating LCN2 in PMN-MDSC. Front Immunol 11:299 (2020). PubMed: 32153594
    • Olinger E  et al. Hepsin-mediated Processing of Uromodulin is Crucial for Salt-sensitivity and Thick Ascending Limb Homeostasis. Sci Rep 9:12287 (2019). PubMed: 31444371
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lipocalin-2 / NGAL antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (kidney)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2016

    Western blot abreview for Anti-Lipocalin-2 / NGAL antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (kidney)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 29 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Lipocalin 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RAW264.7)
    Specification
    RAW264.7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton + 2% BSA in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted Apr 16 2013

    Question

    Dear Sir/Madam,

    I have purchased NGAL primary antibody (AB63929) from your company. I am wondering whether I can use it forimmunoparaffinhistochemistrystaining (IHC-P) or not. I am so thankful if youadvise me. Thank you




    Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 23 2012

    Answer

    Thank you for your inquiry.

    Unfortunately, we do not know if ab63929 is suitable for IHC-P. This has not been experimentally tested by us and is therefore not covered by our guarantee.

    Since you already purchased this antibody, I can suggest to try it in IHC-P and then submit an Abreview to us. We value feedback from our customers even if the result is negative.

    You would earn Abpoints that then can be redeemed as discount of a future purchase or Amazon gift vouchers.

    We do have anti Lipocalin2 antibodies that are tested and guaranteed for IHC-P:

    https://www.abcam.com/index.html?pageconfig=searchresults&search=Lipocalin 2&pt=1&sk=app&sv=74&sn=IHC-P&l=2&fViewMore=1

    I am sorry I could not provide you with precise answer on this occasion and hope this information was nevertheless helpful.

    Read More

    Abcam Scientific Support

    Answered on Jan 23 2012

    Western blot abreview for Anti-Lipocalin 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (urine)
    Loading amount
    5 µg
    Specification
    urine
    Treatment
    CLB (NP-40 based lysis buffer with protease inhibitors)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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