Recombinant Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)
![Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)](https://www.abcam.com/ps/products/216/ab216462/Images/ab216462-290982-anti-lipocalin-2-ngal-antibody-epr21092-western-blot.jpg "Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21092] to Lipocalin-2 / NGAL
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Lipocalin-2 / NGAL antibody [EPR21092]
See all Lipocalin-2 / NGAL primary antibodies -
Description
Rabbit monoclonal [EPR21092] to Lipocalin-2 / NGAL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse bone, white adipose and lung tissue lysates; rat bone tissue lysate. IHC-P: Mouse lung and spleen tissue; rat lung and spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21092 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/2000 dilution recommended for mouse and rat tissues. |
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| WB |
1/1000. Predicted molecular weight: 23 kDa.
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| Notes |
|---|
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. 1/2000 dilution recommended for mouse and rat tissues. |
|
WB
1/1000. Predicted molecular weight: 23 kDa. |
Target
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Database links
- Entrez Gene: 16819 Mouse
- Entrez Gene: 170496 Rat
- SwissProt: P11672 Mouse
- SwissProt: P30152 Rat
- Unigene: 9537 Mouse
- Unigene: 11303 Rat
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Alternative names
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
Images
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Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)All lanes : Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462) at 1/1000 dilution
Lane 1 : Mouse bone tissue lysate at 20 µg
Lane 2 : Mouse white adipose tissue lysate at 20 µg
Lane 3 : Rat bone tissue lysate at 10 µg
Lane 4 : Mouse lung tissue lysate at 10 µg
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile of this antibody is consistent with a recent Nature paper (PMID:28273060).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)](https://www.abcam.com/ps/products/216/ab216462/Images/ab216462-290981-anti-lipocalin-2-ngal-antibody-epr21092-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on mouse lung (PMID: 27136530; PMID: 27729871) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)](https://www.abcam.com/ps/products/216/ab216462/Images/ab216462-290980-anti-lipocalin-2-ngal-antibody-epr21092-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on mouse spleen (PMID: 27729871; PMID: 24587658) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)](https://www.abcam.com/ps/products/216/ab216462/Images/ab216462-290979-anti-lipocalin-2-ngal-antibody-epr21092-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on rat lung (PMID: 27136530; PMID: 27729871) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)](https://www.abcam.com/ps/products/216/ab216462/Images/ab216462-290978-anti-lipocalin-2-ngal-antibody-epr21092-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on rat spleen (PMID: 27729871; PMID: 24587658) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)](https://www.abcam.com/ps/products/216/ab216462/Images/ab216462-6-benefits-of-recombinant-antibodies.png)
Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab216462 has been referenced in 4 publications.
- Majeed Y et al. SIRT1 promotes lipid metabolism and mitochondrial biogenesis in adipocytes and coordinates adipogenesis by targeting key enzymatic pathways. Sci Rep 11:8177 (2021). PubMed: 33854178
- He S et al. Hyperoside protects cardiomyocytes against hypoxia-induced injury via upregulation of microRNA-138. Mol Med Rep 23:N/A (2021). PubMed: 33649812
- Liu X et al. Knockout of NGAL aggravates tubulointerstitial injury in a mouse model of diabetic nephropathy by enhancing oxidative stress and fibrosis. Exp Ther Med 21:321 (2021). PubMed: 33732294
- Jiang DX et al. Prolyl endopeptidase gene disruption attenuates high fat diet-induced nonalcoholic fatty liver disease in mice by improving hepatic steatosis and inflammation. Ann Transl Med 8:218 (2020). PubMed: 32309365