Recombinant
RabMAb

Recombinant Anti-Lipocalin-2 / NGAL antibody [EPR5084] (ab125075)

Overview

  • Product name

    Anti-Lipocalin-2 / NGAL antibody [EPR5084]
    See all Lipocalin-2 / NGAL primary antibodies
  • Description

    Rabbit monoclonal [EPR5084] to Lipocalin-2 / NGAL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Lipocalin-2/ NGAL aa 50-150. The exact sequence is proprietary.
    (Peptide available as ab187960)

  • Positive control

    • Human fetal kidney lysate and SW480 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.Can be blocked with Lipocalin-2 / NGAL peptide (ab187960).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
    • Tissue specificity

      Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the calycin superfamily. Lipocalin family.
    • Cellular localization

      Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
    • Information by UniProt
    • Database links

    • Alternative names

      • 24p3 antibody
      • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
      • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
      • Alpha 2 microglobulin related protein antibody
      • HGNC:6526 antibody
      • HNL antibody
      • Lcn 2 antibody
      • Lcn2 antibody
      • Lipocalin-2 antibody
      • Migration stimulating factor inhibitor antibody
      • MSFI antibody
      • Neutrophil gelatinase associated lipocalin antibody
      • Neutrophil gelatinase associated lipocalin precursor antibody
      • Neutrophil gelatinase-associated lipocalin antibody
      • NGAL antibody
      • NGAL_HUMAN antibody
      • Oncogene 24p3 antibody
      • Oncogenic lipocalin 24p3 antibody
      • p25 antibody
      • Siderocalin antibody
      • siderocalin LCN2 antibody
      • SV40 induced 24P3 protein antibody
      • Uterocalin antibody
      see all

    Images

    • All lanes : Anti-Lipocalin-2 / NGAL antibody [EPR5084] (ab125075) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    References

    This product has been referenced in:

    • Molina L  et al. Hepatocyte-Derived Lipocalin 2 Is a Potential Serum Biomarker Reflecting Tumor Burden in Hepatoblastoma. Am J Pathol 188:1895-1909 (2018). Read more (PubMed: 29920228) »
    • Ku AT  et al. TCF7L1 promotes skin tumorigenesis independently of ß-catenin through induction of LCN2. Elife 6:N/A (2017). WB . Read more (PubMed: 28467300) »
    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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