Anti-Lipocalin-2 / NGAL antibody [29] (ab70287)
Key features and details
- Rat monoclonal [29] to Lipocalin-2 / NGAL
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Lipocalin-2 / NGAL antibody [29]
See all Lipocalin-2 / NGAL primary antibodies -
Description
Rat monoclonal [29] to Lipocalin-2 / NGAL -
Host species
Rat -
Specificity
ab70287 binds Lipocalin-2 / NGAL in mouse serum. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab70287 is protein A/G purified. -
Clonality
Monoclonal -
Clone number
29 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration.
Microwave pretreatment using citrate buffer pH 6, is recommended. |
Notes |
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IHC-P
Use at an assay dependent concentration. Microwave pretreatment using citrate buffer pH 6, is recommended. |
Target
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Database links
- Entrez Gene: 16819 Mouse
- SwissProt: P11672 Mouse
- Unigene: 9537 Mouse
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Alternative names
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab70287 has been referenced in 8 publications.
- Raghavan R et al. Plakophilin3 loss leads to increased adenoma formation and rectal prolapse in APCmin mice. Biochem Biophys Res Commun 586:14-19 (2022). PubMed: 34823217
- Jo J et al. Protective Effects of Orexin A in a Murine Model of Cisplatin-Induced Acute Kidney Injury. J Clin Med 11:N/A (2022). PubMed: 36498769
- Li H et al. Tubular β-catenin alleviates mitochondrial dysfunction and cell death in acute kidney injury. Cell Death Dis 13:1061 (2022). PubMed: 36539406
- Chiba T et al. Endothelial-Derived miR-17∼92 Promotes Angiogenesis to Protect against Renal Ischemia-Reperfusion Injury. J Am Soc Nephrol 32:553-562 (2021). PubMed: 33514560
- Kholia S et al. Human Liver Stem Cell Derived Extracellular Vesicles Alleviate Kidney Fibrosis by Interfering with the β-Catenin Pathway through miR29b. Int J Mol Sci 22:N/A (2021). PubMed: 34639119
- Ni Y et al. Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI. Theranostics 11:9431-9451 (2021). PubMed: 34646379
- Shim YJ et al. Early T cell infiltration is modulated by programed cell death-1 protein and its ligand (PD-1/PD-L1) interactions in murine kidney transplants. Kidney Int 98:897-905 (2020). PubMed: 32763116
- Chiba T et al. Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI. J Am Soc Nephrol 30:2384-2398 (2019). PubMed: 31575700