Key features and details
- Rat monoclonal  to Lipocalin-2 / NGAL
- Suitable for: WB, ELISA, IHC-P, Sandwich ELISA
- Reacts with: Mouse
- Isotype: IgG1
Product nameAnti-Lipocalin-2 / NGAL antibody 
See all Lipocalin-2 / NGAL primary antibodies
DescriptionRat monoclonal  to Lipocalin-2 / NGAL
Specificityab70287 binds Lipocalin-2 / NGAL in mouse serum.
Tested applicationsSuitable for: WB, ELISA, IHC-P, Sandwich ELISAmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse Lipocalin-2/ NGAL. Recombinant mouse Lipocalin-2/ NGAL adsorbed onto aluminum hydroxide gel
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab70287 is protein A/G purified.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab70287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.
Microwave pretreatment using citrate buffer pH 6, is recommended.
|Sandwich ELISA||Use at an assay dependent concentration. Can be used as Detection antibody with recommended pair.|
FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Information by UniProt
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
ab70287 has not yet been referenced specifically in any publications.