Overview

  • Product name
    Anti-Lipocalin-2 / NGAL antibody [5G5]
    See all Lipocalin-2 / NGAL primary antibodies
  • Description
    Mouse monoclonal [5G5] to Lipocalin-2 / NGAL
  • Host species
    Mouse
  • Specificity
    ab23477 is specific for Lipocalin-2 / NGAL, native and denatured forms.
  • Tested applications
    Suitable for: IHC-P, ELISA, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Lipocalin-2/ NGAL. Full length native human neutrophil gelatinase associated lipocalin isolated from neutrophils.

  • Positive control
    • Capa-2, BxPC-3, ASPC1 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab23477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10. PubMed: 18317594
ELISA 1/30000.
IHC-FoFr 1/10.
WB 1/1000. Detects a band of approximately 25-35 kDa (predicted molecular weight: 23 kDa). Non reducing conditions will give multiple bands of 35, 70 and 55 kDa. Do not use serum for blocking as serum will contain Lipocalin 2

Target

  • Function
    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity
    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization
    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • Database links
  • Alternative names
    • 24p3 antibody
    • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
    • Alpha 2 microglobulin related protein antibody
    • HGNC:6526 antibody
    • HNL antibody
    • Lcn 2 antibody
    • Lcn2 antibody
    • Lipocalin-2 antibody
    • Migration stimulating factor inhibitor antibody
    • MSFI antibody
    • Neutrophil gelatinase associated lipocalin antibody
    • Neutrophil gelatinase associated lipocalin precursor antibody
    • Neutrophil gelatinase-associated lipocalin antibody
    • NGAL antibody
    • NGAL_HUMAN antibody
    • Oncogene 24p3 antibody
    • Oncogenic lipocalin 24p3 antibody
    • p25 antibody
    • Siderocalin antibody
    • siderocalin LCN2 antibody
    • SV40 induced 24P3 protein antibody
    • Uterocalin antibody
    see all

Images

  • ab23477 staining human skin tissue sections by IHC-P.  Sections were fixed in 10% neutral buffered formalin and subjected to heat mediated antigen retrieval prior to blocking with 1% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/50 and incubated with the sample for 20 hours at 4°C.  A biotinylated horse anti-mouse antibody diluted 1/200 was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human PaCa tissue sections labelling Lipocalin 2 with ab23477.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human chronic pancreatits tissue sections labelling Lipocalin-2 / NGAL with ab23477.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human pancreas tissue sections labelling Lipocalin-2 / NGAL with ab23477.

References

This product has been referenced in:
  • Ku AT  et al. TCF7L1 promotes skin tumorigenesis independently of ß-catenin through induction of LCN2. Elife 6:N/A (2017). Read more (PubMed: 28467300) »
  • Wang X  et al. iTRAQ-Based Proteomics Screen identifies LIPOCALIN-2 (LCN-2) as a potential biomarker for colonic lateral-spreading tumors. Sci Rep 6:28600 (2016). WB, IHC . Read more (PubMed: 27339395) »
See all 12 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

DISCOUNT CODE: ***********
Expiration date: ***********
I am very pleased to hear you would like to accept our offer and test ab23477 in Rhesus Monkey. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Rhesus Monkey and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your reply.
As mentioned in the previous email, to take advantage of the testing discount please let me know and I will send you a testing discount code. After you receive the discount code, please follow the steps below:
1 - Place order for ab23477, you will pay full price + shipping and handling.
2 - Test the antibody on rhesus monkey samples.
3 - Write and Abreview on how tha ntibody worked, it can be positive or negative.
4 - Once the Abreview is submitted, then the testing discount code that I originally sent will become active. You can then use that code to receive one free primary antibody of your choice.
I hope that helps explain how the process works, you can find more information in the following link:
https://www.abcam.com/index.htmlpageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
Please let me know if you would like me to send you the testing discount code.
I look forward to your response.

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Answer

Thank you very much for your interest in ab23477.
We do not provide sample sizes of our antibodies and since ab23477 has not been tested in rhesus monkey, I can offer a discount off a future purchase if you buy ab23477 now, test it in rhesus monkey and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab(xxx) in rhesus monkey. I will then send a discount code. This code must be issued before purchasing ab23477 so please wait for my reply before ordering.
2. Purchase ab23477 either by phone, fax, or online (www.abcam.com).
3. Test it in rhesus monkey.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab23477 turns out to be unsuitable for rhesus monkey, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human sebacous gland cells)
Loading amount
60 µg
Specification
human sebacous gland cells
Treatment
13-cis Retinoic acid for 48 hours
Gel Running Conditions
Reduced Denaturing (4- 12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human skin sections)
Specification
Human skin sections
Fixative
10% Neutral Buffered Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trilogy (Cell Marque)
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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