Anti-Lipocalin-2 / NGAL antibody (ab89819)
Key features and details
- Mouse polyclonal to Lipocalin-2 / NGAL
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Lipocalin-2 / NGAL antibody
See all Lipocalin-2 / NGAL primary antibodies -
Description
Mouse polyclonal to Lipocalin-2 / NGAL -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Lipocalin-2/ NGAL aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_005555.1 -
Positive control
- WB: spleen tissue lysate. IHC: pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IHC-P |
Notes |
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WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Database links
- Entrez Gene: 3934 Human
- Omim: 600181 Human
- SwissProt: P80188 Human
- Unigene: 204238 Human
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Alternative names
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
Images
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Anti-Lipocalin-2 / NGAL antibody (ab89819) at 1 µg/ml + Human spleen tissue lysate at 50 µg
Predicted band size: 23 kDa
Observed band size: 23 kDa -
All lanes : Anti-Lipocalin-2 / NGAL antibody (ab89819) at 1 µg/ml
Lane 1 : Lipocalin 2 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: ~60 kDa. We are unsure as to the identity of these extra bands. -
ab89819 at 3µg/ml staining Lipocalin-2/ NGAL in formalin-fixed, paraffin-embedded Human pancreas tissue by immunohistochemistry .
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab89819 has not yet been referenced specifically in any publications.