Overview

  • Product name

    Anti-Lipocalin-2 / NGAL antibody
    See all Lipocalin-2 / NGAL primary antibodies
  • Description

    Mouse polyclonal to Lipocalin-2 / NGAL
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Lipocalin-2/ NGAL aa 1-198.
    Database link: NP_005555.1

  • Positive control

    • WB: spleen tissue lysate. IHC: pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab89819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
    • Tissue specificity

      Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the calycin superfamily. Lipocalin family.
    • Cellular localization

      Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
    • Information by UniProt
    • Database links

    • Alternative names

      • 24p3 antibody
      • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
      • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
      • Alpha 2 microglobulin related protein antibody
      • HGNC:6526 antibody
      • HNL antibody
      • Lcn 2 antibody
      • Lcn2 antibody
      • Lipocalin-2 antibody
      • Migration stimulating factor inhibitor antibody
      • MSFI antibody
      • Neutrophil gelatinase associated lipocalin antibody
      • Neutrophil gelatinase associated lipocalin precursor antibody
      • Neutrophil gelatinase-associated lipocalin antibody
      • NGAL antibody
      • NGAL_HUMAN antibody
      • Oncogene 24p3 antibody
      • Oncogenic lipocalin 24p3 antibody
      • p25 antibody
      • Siderocalin antibody
      • siderocalin LCN2 antibody
      • SV40 induced 24P3 protein antibody
      • Uterocalin antibody
      see all

    Images

    • Anti-Lipocalin-2 / NGAL antibody (ab89819) at 1 µg/ml + Human spleen tissue lysate at 50 µg

      Predicted band size: 23 kDa
      Observed band size: 23 kDa

    • All lanes : Anti-Lipocalin-2 / NGAL antibody (ab89819) at 1 µg/ml

      Lane 1 : Lipocalin 2 transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 23 kDa
      Observed band size: 23 kDa
      Additional bands at: ~60 kDa. We are unsure as to the identity of these extra bands.

    • ab89819 at 3µg/ml staining Lipocalin-2/ NGAL in formalin-fixed, paraffin-embedded Human pancreas tissue by immunohistochemistry .

    References

    ab89819 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab89819.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up