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Immunology Adaptive Immunity T Cells Non-CD
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Anti-Lipocalin-2 / NGAL antibody [ABS 039-08] (ab53500)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [ABS 039-08] (ab53500)

    Key features and details

    • Mouse monoclonal [ABS 039-08] to Lipocalin-2 / NGAL
    • Suitable for: IHC-P
    • Reacts with: Rat
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
    Recombinant Mouse Lipocalin-2 / NGAL protein (ab95008)

    View more associated products

    Overview

    • Product name

      Anti-Lipocalin-2 / NGAL antibody [ABS 039-08]
      See all Lipocalin-2 / NGAL primary antibodies
    • Description

      Mouse monoclonal [ABS 039-08] to Lipocalin-2 / NGAL
    • Host species

      Mouse
    • Specificity

      This antibody reacts with Lipocalin-2 / NGAL
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Rat
      Does not react with: Human
    • Immunogen

      Recombinant full length protein (His-tag) corresponding to Rat Lipocalin-2/ NGAL (C terminal). His-tag coupled to carrier

    • General notes

      FUSION PARTNER: Sp2/mIL-6.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituents: 2.9% Sodium chloride, 0.0268% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      ABS 039-08
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • Non-CD
      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Binding proteins
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cancer
      • Tumor biomarkers
      • Other
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Mouse Lipocalin-2 / NGAL protein (ab95008)

    Applications

    Our Abpromise guarantee covers the use of ab53500 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use at an assay dependent concentration.

    Target

    • Function

      Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
    • Tissue specificity

      Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the calycin superfamily. Lipocalin family.
    • Cellular localization

      Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
    • Target information above from: UniProt accession P80188 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 170496 Rat
      • SwissProt: P30152 Rat
      • Unigene: 11303 Rat
      • Alternative names

        • 24p3 antibody
        • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
        • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
        • Alpha 2 microglobulin related protein antibody
        • HGNC:6526 antibody
        • HNL antibody
        • Lcn 2 antibody
        • Lcn2 antibody
        • Lipocalin-2 antibody
        • Migration stimulating factor inhibitor antibody
        • MSFI antibody
        • Neutrophil gelatinase associated lipocalin antibody
        • Neutrophil gelatinase associated lipocalin precursor antibody
        • Neutrophil gelatinase-associated lipocalin antibody
        • NGAL antibody
        • NGAL_HUMAN antibody
        • Oncogene 24p3 antibody
        • Oncogenic lipocalin 24p3 antibody
        • p25 antibody
        • Siderocalin antibody
        • siderocalin LCN2 antibody
        • SV40 induced 24P3 protein antibody
        • Uterocalin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [ABS 039-08] (ab53500)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [ABS 039-08] (ab53500)

        Lipocalin-2 / NGAL immunostaining in kidney, Formalin-fixed, paraffin-embedded tissue sections of cortical epithelial cells in the S1/S2 segments (proximal convoluted tubules) following DXR-induced acute kidney injury. Top panel, saline treatment; bottom panel, DXR treatment; X630 (2).

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab53500? Please let us know so that we can cite the reference in this datasheet.

      ab53500 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Customer kindly called us today to inquire about the specific protocol used for the image on the datasheet.

      Was there an antigen retrieval step? If so, which system was used?

      What is the concentration of antibody used in the experiments in this image?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 14 2012

      Answer

      I was able to obtain the protocol used by the scientists who made the images on the datasheet. Antigen retrieval is required and primary antibody incubation was done at 1:50. I have attached this protocol in pdf format for your review.

      Read More

      Abcam Scientific Support

      Answered on Dec 14 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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