• Product name

    Lipolysis Assay Kit (Colorimetric)
    See all Lipolysis kits
  • Detection method

  • Sample type

    Cell culture supernatant
  • Assay type

  • Sensitivity

    < 0.2 nmol/well
  • Range

    2 nmol/well - 10 nmol/well
  • Assay time

    0h 40m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Abcam's Lipolysis Assay Kit (Colorimetric) (ab185433) is simple and easy-to-use. The assay measures glycerol released from 3T3-L1 cells after induction of lipolysis using colorimetric method. The color intensity is directly proportional to the amount of glycerol. This assay kit can detect less than 200 pmol of glycerol.

    Lipolysis assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - incubate for 30 min
    - analyze with microplate reader

  • Notes

    Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamine and certain hormones. The kit includes synthetic catecholamine, Isoproterenol, which activates β-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin.

  • Platform

    Microplate reader


  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Glycerol Assay Buffer WM 1 x 25ml
    Glycerol Enzyme Mix Green 1 vial
    Glycerol Probe 1 x 0.2ml
    Glycerol Standard (100 mM) Yellow 1 x 0.2ml
    Isoproterenol (10mM) 1 x 50µl
    Lipolysis Assay Buffer 1 x 17ml
    Lipolysis Wash Buffer 1 x 22ml
  • Research areas


  • 50 μl of 3T3-L1 cells treated with vehicle control (H2O) or 100 nM Isoproterenol for 0-3 hours.



This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

I tried many different products to measure Lipolysis in adipocyte cells better and clearer but failed then I bought Lipolysis Assay Kit (Colorimetric) (ab185433)) to measure lipolysis in 3T3L1 adipocyte cells, as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best product to detect insulin resistance during in vivo and in vitro studies

Dr. Shailendra Singh

Verified customer

Submitted Aug 16 2018

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