Recombinant Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
![Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)](https://www.abcam.com/ps/products/240/ab240014/Images/ab240014-408198-anti-lipoprotein-a-antibody-epr6474-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6474] to Lipoprotein a - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free
See all Lipoprotein a primary antibodies -
Description
Rabbit monoclonal [EPR6474] to Lipoprotein a - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Lipoprotein a aa 150-250. The exact sequence is proprietary.
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General notes
ab240014 is the carrier-free version of ab125014 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240014 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6474 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab240014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 501 kDa (predicted molecular weight: 501 kDa). |
Target
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Relevance
Lipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis. -
Database links
- Entrez Gene: 4018 Human
- Omim: 152200 Human
- SwissProt: P08519 Human
- Unigene: 520120 Human
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Alternative names
- AK38 antibody
- Antiangiogenic AK38 protein antibody
- apo(a) antibody
see all
Images
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Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/50000 dilution (purified) + Human plasma at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 501 kDa
Observed band size: 501 kDaThis data was developed using ab125014, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed bands are consistant with desciption in PMID:10703674, which should be N-terminal fragments.
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![Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)](https://www.abcam.com/ps/products/240/ab240014/Images/ab240014-406315-anti-lipoprotein-a-antibody-epr6474-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/20000 dilution (unpurified) + Human plasma lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 501 kDa
Observed band size: 500 kDa why is the actual band size different from the predicted?This data was developed using ab125014, the same antibody clone in a different buffer formulation.
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![Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)](https://www.abcam.com/ps/products/240/ab240014/Images/ab240014-3-benefits-of-recombinant-antibodies.png)
Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab240014 has not yet been referenced specifically in any publications.
