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    lipoprotein-a-antibody-epr6474-bsa-and-azide-free-ab240014.pdf

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RecombinantRabMAb

Recombinant Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
  • Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
  • Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6474] to Lipoprotein a - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)

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Overview

  • Product name

    Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free
    See all Lipoprotein a primary antibodies
  • Description

    Rabbit monoclonal [EPR6474] to Lipoprotein a - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Lipoprotein a aa 150-250. The exact sequence is proprietary.

  • General notes

    ab240014 is the carrier-free version of ab125014 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240014 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6474
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Lipoproteins
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Lipoprotein a antibody [EPR6474] (ab125014)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab240014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 501 kDa (predicted molecular weight: 501 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Relevance

      Lipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis.
    • Database links

      • Entrez Gene: 4018 Human
      • Omim: 152200 Human
      • SwissProt: P08519 Human
      • Unigene: 520120 Human
      • Alternative names

        • AK38 antibody
        • Antiangiogenic AK38 protein antibody
        • apo(a) antibody
        • APOA antibody
        • apolipoprotein(a) antibody
        • LP antibody
        • lp(a) antibody
        • LPA antibody
        see all

      Images

      • Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
        Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
        Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/50000 dilution (purified) + Human plasma at 10 µg

        Secondary
        HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

        Predicted band size: 501 kDa
        Observed band size: 501 kDa



        This data was developed using ab125014, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

        The observed bands are consistant with desciption in PMID:10703674, which should be N-terminal fragments.

      • Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
        Western blot - Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
        Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/20000 dilution (unpurified) + Human plasma lysate at 10 µg

        Secondary
        HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 501 kDa
        Observed band size: 500 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab125014, the same antibody clone in a different buffer formulation.

      • Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)
        Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free (ab240014)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab240014? Please let us know so that we can cite the reference in this datasheet.

      ab240014 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab240014.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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