Anti-Lipoprotein lipase antibody [5D2] (ab93898)
![Immunocytochemistry - Anti-Lipoprotein lipase antibody [5D2] (ab93898)](https://www.abcam.com/ps/products/93/ab93898/Images/ab93898-3-anti-Lipoprotein-lipase-antibody-5D2-immunocytochemistry-immunofluorescence-HeLa-human.jpg "Immunocytochemistry - Anti-Lipoprotein lipase antibody [5D2] (ab93898)")
Key features and details
- Mouse monoclonal [5D2] to Lipoprotein lipase
- Suitable for: ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Cat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Lipoprotein lipase antibody [5D2]
See all Lipoprotein lipase primary antibodies -
Description
Mouse monoclonal [5D2] to Lipoprotein lipase -
Host species
Mouse -
Specificity
This antibody does not recognise hepatic lipase. -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Cat, Human -
Immunogen
Full length native protein (purified) corresponding to Cow Lipoprotein lipase. Purified bovine milk lipoprotein lipase protein.
Database link: P11151 -
Positive control
- ICC: HeLa cells and mouse cortical glia. Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5D2 -
Isotype
IgG1 -
Research areas
Associated products
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Assay kits
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Coimmunogen
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 - 5 µg/ml.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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ICC/IF
Use a concentration of 2 - 5 µg/ml. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. -
Involvement in disease
Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
Post-translational
modificationsTyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. -
Cellular localization
Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 4023 Human
- Entrez Gene: 16956 Mouse
- Omim: 609708 Human
- SwissProt: P06858 Human
- SwissProt: P11152 Mouse
- Unigene: 180878 Human
- Unigene: 1514 Mouse
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Alternative names
- EC 3.1.1 antibody
- EC 3.1.1.34 antibody
- HDLCQ11 antibody
see all
Images
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Immunocytochemistry - Anti-Lipoprotein lipase antibody [5D2] (ab93898)ab93898 staining Lipoprotein lipase in wild-type HeLa cells (top panel) and LPL knockout HeLa cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab93898 at 5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Flow Cytometry - Anti-Lipoprotein lipase antibody [5D2] (ab93898)](https://www.abcam.com/ps/products/93/ab93898/Images/ab93898-108863-anti-lipoprotein-lipase-antibody-5d2-flow-cytometry.jpg)
Flow Cytometry - Anti-Lipoprotein lipase antibody [5D2] (ab93898)Overlay histogram showing HeLa cells stained with ab93898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93898, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (ab93898)](https://www.abcam.com/ps/products/93/ab93898/Images/ab93898-233401-anti-lipoprotein-lipase-antibody-5d2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (ab93898)Immunocytochemistry/Immunofluorescence analysis of mouse cortical glia labelling Lipoprotein lipase (green) with ab93898 at 2µg/ml. Cells were permemabilized with PBS + 0.1% Triton X-100 and blocked with PBS + 10% FBS. Nuclei stained with Hoechst (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab93898 has been referenced in 4 publications.
- Sharoar MG et al. Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains. Mol Neurodegener 16:45 (2021). PubMed: 34215298
- Wang Y et al. MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase. Yonsei Med J 60:1187-1194 (2019). PubMed: 31769250
- Graham BB et al. Severe pulmonary hypertension is associated with altered right ventricle metabolic substrate uptake. Am J Physiol Lung Cell Mol Physiol 309:L435-40 (2015). WB, IHC-P ; Rat . PubMed: 26115672
- Chang SF et al. Detailed characterization of the binding site of the lipoprotein lipase-specific monoclonal antibody 5D2. J Lipid Res 39:2350-9 (1998). PubMed: 9831623