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Thank you for your call earlier this week and for your patience while I have been in touch with the lab.
Ab93898 has been cited for use in Western blot in the literature, but we don't have an in-house protocol.I've attached an article that describes the protocol used (see page 2352). It looks like the authorsalso used 1 ug/mL concentration so I think that is fine for you to continue using. Their secondary antibody was diluted 1:50,000, and the optimal secondary antibody dilution can definitely vary but it might be beneficial to dilute yours further. I noticed that the authors used milk to block, but if the no-protein block is working well then I would recommend continuing to use it.
I hope this information will be useful, but please let me know if there is anything else that we can do for you, and keep me updated of any progress with the results.
Answered on May 10 2012