Anti-Lipoprotein lipase antibody (ab217200)

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Overview

  • Product name

    Anti-Lipoprotein lipase antibody
    See all Lipoprotein lipase primary antibodies

  • Description

    Rabbit polyclonal to Lipoprotein lipase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human Lipoprotein lipase aa 20-70 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    ASRGGVAAADQ RRDFIDIESK FALRTPEDTA EDTCHLIPGV AESVATCHFN


    Database link: P06858

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Rat small intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab217200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.

  • Involvement in disease

    Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.

  • Sequence similarities

    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.

  • Post-translational
    modifications

    Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

  • Cellular localization

    Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 3.1.1 antibody
    • EC 3.1.1.34 antibody
    • HDLCQ11 antibody
    • LIPD antibody
    • LIPL_HUMAN antibody
    • Lipoprotein lipase antibody
    • LPL antibody
    • LPL protein antibody
    • MGC137861 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody (ab217200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody (ab217200)

    Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat small intestine tissue labeling Lipoprotein lipase with ab217200 at 1/400 dilution followed by a conjugated secondary and DAB staining.

References

ab217200 has not yet been referenced specifically in any publications.

Publishing research using ab217200? Please let us know so that we can cite the reference in this datasheet.

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