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    lipoprotein-lipase-antibody-epr15552-ab172953.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Lipases
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RecombinantRabMAb

Recombinant Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

  • Datasheet
  • SDS
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Western blot - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
  • Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1555(2)] to Lipoprotein lipase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Lipoprotein lipase antibody [EPR1555(2)]
    See all Lipoprotein lipase primary antibodies
  • Description

    Rabbit monoclonal [EPR1555(2)] to Lipoprotein lipase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Cat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, fetal heart, pancreas and fetal liver lysates. Human liver and pancreas tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1555(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-Lipoprotein lipase antibody [EPR1555(2)] - BSA and Azide free (ab249731)
  • Assay kits

    • Lipoprotein Lipase Assay Kit (Fluorometric) (ab204721)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Lipoprotein lipase (ab115504)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 53 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 53 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
  • Involvement in disease

    Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.
  • Sequence similarities

    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • Post-translational
    modifications

    Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
  • Cellular localization

    Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Target information above from: UniProt accession P06858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4023 Human
    • Omim: 609708 Human
    • SwissProt: P06858 Human
    • Unigene: 180878 Human
    • Alternative names

      • EC 3.1.1 antibody
      • EC 3.1.1.34 antibody
      • HDLCQ11 antibody
      • LIPD antibody
      • LIPL_HUMAN antibody
      • Lipoprotein lipase antibody
      • LPL antibody
      • LPL protein antibody
      • MGC137861 antibody
      see all

    Images

    • Western blot - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
      Western blot - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
      All lanes : Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human pancreas lysate
      Lane 4 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
      Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab172953? Please let us know so that we can cite the reference in this datasheet.

    ab172953 has been referenced in 1 publication.

    • Shen J  et al. MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition. Aging (Albany NY) 12:15756-15770 (2020). PubMed: 32805717

    Customer reviews and Q&As

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