RecombinantRabMAb

Recombinant Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1555(2)] to Lipoprotein lipase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Lipoprotein lipase antibody [EPR1555(2)]
    See all Lipoprotein lipase primary antibodies

  • Description

    Rabbit monoclonal [EPR1555(2)] to Lipoprotein lipase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Cat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Lipoprotein lipase aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P06858

  • Positive control

    • Human fetal kidney, fetal heart, pancreas and fetal liver lysates. Human liver and pancreas tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab172953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 53 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function

      The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.

    • Involvement in disease

      Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.

    • Sequence similarities

      Belongs to the AB hydrolase superfamily. Lipase family.
      Contains 1 PLAT domain.

    • Post-translational
      modifications

      Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

    • Cellular localization

      Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
    • Information by UniProt

    • Database links

      • Alternative names

        • EC 3.1.1 antibody
        • EC 3.1.1.34 antibody
        • HDLCQ11 antibody
        • LIPD antibody
        • LIPL_HUMAN antibody
        • Lipoprotein lipase antibody
        • LPL antibody
        • LPL protein antibody
        • MGC137861 antibody
        see all

      Images

      • Western blot - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
        Western blot - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
        All lanes : Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953) at 1/10000 dilution

        Lane 1 : Human fetal kidney lysate
        Lane 2 : Human fetal heart lysate
        Lane 3 : Human pancreas lysate
        Lane 4 : Human fetal liver lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 53 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

        Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)

        Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References (0)

      Publishing research using ab172953? Please let us know so that we can cite the reference in this datasheet.

      ab172953 has not yet been referenced specifically in any publications.

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