Recombinant Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Lipoprotein lipase antibody [EPR1555(2)]
See all Lipoprotein lipase primary antibodies -
Description
Rabbit monoclonal [EPR1555(2)] to Lipoprotein lipase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Cat
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Lipoprotein lipase aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P06858 -
Positive control
- Human fetal kidney, fetal heart, pancreas and fetal liver lysates. Human liver and pancreas tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1555(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab172953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 53 kDa. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. -
Involvement in disease
Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
Post-translational
modificationsTyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. -
Cellular localization
Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 4023 Human
- Omim: 609708 Human
- SwissProt: P06858 Human
- Unigene: 180878 Human
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Alternative names
- EC 3.1.1 antibody
- EC 3.1.1.34 antibody
- HDLCQ11 antibody
see all
Images
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![Western blot - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)](https://www.abcam.com/ps/products/172/ab172953/Images/ab172953-196094-anti-lipoprotein-lipase-antibody-epr15552-western-blot.jpg)
Western blot - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)All lanes : Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human pancreas lysate
Lane 4 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)](https://www.abcam.com/ps/products/172/ab172953/Images/ab172953-196099-anti-lipoprotein-lipase-antibody-epr15552-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)](https://www.abcam.com/ps/products/172/ab172953/Images/ab172953-196097-anti-lipoprotein-lipase-antibody-epr15552-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.
Datasheets and documents
References
ab172953 has not yet been referenced specifically in any publications.
