Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)

Reviews (6)Q&A (11)References (26)

Overview

  • Product name
    Anti-Lipoprotein lipase antibody [LPL.A4]
    See all Lipoprotein lipase primary antibodies
  • Description
    Mouse monoclonal [LPL.A4] to Lipoprotein lipase
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Full length protein (Human).

  • Positive control
    • Purified human and bovine recombinant Lipoprotein lipase.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab21356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21062953
IHC-P Use at an assay dependent concentration. PubMed: 20200316
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 53 kDa.
ELISA 1/2000 - 1/5000.

Target

  • Function
    The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
  • Involvement in disease
    Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.
  • Sequence similarities
    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • Post-translational
    modifications
    Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
  • Cellular localization
    Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • EC 3.1.1 antibody
    • EC 3.1.1.34 antibody
    • HDLCQ11 antibody
    • LIPD antibody
    • LIPL_HUMAN antibody
    • Lipoprotein lipase antibody
    • LPL antibody
    • LPL protein antibody
    • MGC137861 antibody
    see all

Images

  • Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
    Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
    All lanes : Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

    Lane 1 : Human Lipoprotein lipase.
    Lane 2 : Bovine Lipoprotein lipase

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 53 kDa
    Observed band size: 55,60 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)Image is courtesy of an anonymous AbReview

    ab21356 staining Lipoprotein lipase in Mouse small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 30 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 ug/ml) for 14 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
    Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
    Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
    Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)Image is courtesy of an anonymous AbReview

    ab21356 staining Lipoprotein lipase in Mouse intestine cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 23°C. Samples were incubated with primary antibody (1/500) for 8 hours at 4°C. An Alexa Fluor® 488-conjugated Rat anti-mouse IgG1 polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Gong D  et al. Coiled-coil domain-containing 80 accelerates atherosclerosis development through decreasing lipoprotein lipase expression via ERK1/2 phosphorylation and TET2 expression. Eur J Pharmacol 843:177-189 (2019). Read more (PubMed: 30439364) »
  • Ruiz-Palacios M  et al. Insulin Treatment May Alter Fatty Acid Carriers in Placentas from Gestational Diabetes Subjects. Int J Mol Sci 18:N/A (2017). WB . Read more (PubMed: 28587267) »
See all 26 Publications for this product

Publishing research using ab21356? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 17 Abreviews or Q&A

Question

Can you tell me if the epitope of this Ab is located in an extracellular, cytoplasmatic or transmembrane region of the LPL protein.

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Answer

This antibody was raised against the whole Human LPL whole protein as stated in the datasheet.

Unfortunately, the exact epitope is unknown as the whole protein with all regions intact was used to produce the antibody and the epitope has not been mapped.

Read More

Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Sep 11 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5µg/mL · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citra Plus
Sample
Mouse Tissue sections (Small Intestine)
Specification
Small Intestine
Permeabilization
No
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 28 2013

Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
30 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Mr. Dongil Kim

Verified customer

Submitted Apr 11 2013

Question

Yes, that’s my order. A credit would be greatly appreciated. The conc we used was 1:10,000.

Thanks

Read More
Answer

Thank you for your reply and for confirming those details.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting departmentat us.credits@abcam.com or by telephone at 888-772-2226. Please refer to the credit note ID in any correspondence with our accounting department.

Please let me know if there is anything else that we can do for you and I'll be happy to help. Thank you again for your patience in resolving these issues.

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Question

Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

Thanks so much for your help.

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Answer

One more thing. Could you please tell me what dilution you've tried with this secondary antibody, for our records?

Thank you so much!

Read More

Question

Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

Thanks so much for your help.

Read More
Answer

Thank you for your reply.

I have recorded all of the information so that we can further investigate the trouble with the antibody. I again apologize that it is not working as expecting, but I'm glad you've found a way to get some results.

With the ab98693, I've found an order on the PO#***. Can you confirm that this is your order? I will process the credit or refund, whichever you prefer.

I look forward to hearing from you. Please let me know if you have any further questions or if there is anything else that we can do for you.

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Question

I apologize to replying this late. We did more exp and found that we really need to use more antibody to “see” the bands on the membrane. So the best concentration for this antibody is 1:250 and overnight incubation at 4degC. Did you manage to retrieve the QC data for this antibody?

As for the secondary, the abcam one actually did not work. Thanks again for your help

Read More
Answer

Thank you for your reply and for keeping me updated.

I am glad to hear that some bands were seen on the membrane, but I am sorry that it took such a high concentration in order to work in this assay. I have only been able to get the QC data for the first lot that you used, which is attached to this email. The left lane shows human LPL and the right lane shows bovine LPL. The QC data for the new lot is not available at this time.

Which secondary antibody did not work? I will be happy to refund or replace this antibody if you would like.

I look forward to hearing from you. Please let me know if you have further questions or if there is anything else that we can do.

Read More

Question

I have attached the slides of our data using A4 anti-LPL. Let me know if you need further info.

Thanks

Read More
Answer

Thank youagain for letting us know about the trouble with this antibody.

Have you had a chance to try the replacement vial of ab21356 from a new lot, or done any other testing?

I have received the QC data from the lab for the first lot that you tried, but the QC data for the new lot is unfortunately not available. Please see the data attached. Lane 1 is human LPL and lane 2 is bovine LPL.

I look forward to hearing from you. If there is anything else that we can do for you please let me know.

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Question

I have attached the slides of our data using A4 anti-LPL. Let me know if you need further info.



Thanks

Read More
Answer

Thank you so much for forwarding this data.

I will let you know once I havefurther informationfrom the lab. Please keep me updated about new results with the new vial.

I look forward to hearing from you and hope you're having a great day!

Read More

1-10 of 17 Abreviews or Q&A

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