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Full length protein (Human).
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab21356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21062953|
|IHC-P||Use at an assay dependent concentration. PubMed: 20200316|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 53 kDa.|
|ELISA||1/2000 - 1/5000.|
ab21356 staining Lipoprotein lipase in Mouse small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 30 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 ug/ml) for 14 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
ab21356 staining Lipoprotein lipase in Mouse intestine cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 23°C. Samples were incubated with primary antibody (1/500) for 8 hours at 4°C. An Alexa Fluor® 488-conjugated Rat anti-mouse IgG1 polyclonal (1/250) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"