For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    lipoprotein-lipase-antibody-lpla4-ab21356.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Lipids / Lipoproteins Lipid Metabolism Lipases
Share by email

Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)

  • Datasheet
  • SDS
Reviews (8)Q&A (11)References (45)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
  • Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)

Key features and details

  • Mouse monoclonal [LPL.A4] to Lipoprotein lipase
  • Suitable for: Flow Cyt, WB
  • Reacts with: Cow, Human
  • Isotype: IgG1

You may also be interested in

Primary
Product image
Anti-CPT1B antibody [EPR7838] (ab134135)
Biochemical
Product image
PDTC (Ammonium pyrrolidinedithiocarbamate), NF-kappaB inhibitor antioxidant (ab141406)
Assay
Product image
Lipoprotein Lipase Assay Kit (Fluorometric) (ab204721)

View more associated products

Overview

  • Product name

    Anti-Lipoprotein lipase antibody [LPL.A4]
    See all Lipoprotein lipase primary antibodies
  • Description

    Mouse monoclonal [LPL.A4] to Lipoprotein lipase
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Cow, Human
  • Immunogen

    Full length protein corresponding to Human Lipoprotein lipase.

  • Positive control

    • Purified human and bovine recombinant Lipoprotein lipase.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A affinity chromatography
  • Clonality

    Monoclonal
  • Clone number

    LPL.A4
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Assay kits

    • Lipoprotein Lipase Assay Kit (Fluorometric) (ab204721)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Lipoprotein lipase (ab115504)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (4)
1/1000. Predicted molecular weight: 53 kDa.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/1000. Predicted molecular weight: 53 kDa.

Target

  • Function

    The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
  • Involvement in disease

    Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.
  • Sequence similarities

    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • Post-translational
    modifications

    Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
  • Cellular localization

    Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Target information above from: UniProt accession P06858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280843 Cow
    • Entrez Gene: 4023 Human
    • Omim: 609708 Human
    • SwissProt: P11151 Cow
    • SwissProt: P06858 Human
    • Unigene: 180878 Human
    • Alternative names

      • EC 3.1.1 antibody
      • EC 3.1.1.34 antibody
      • HDLCQ11 antibody
      • LIPD antibody
      • LIPL_HUMAN antibody
      • Lipoprotein lipase antibody
      • LPL antibody
      • LPL protein antibody
      • MGC137861 antibody
      see all

    Images

    • Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      All lanes : Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

      Lane 1 : Human Lipoprotein lipase.
      Lane 2 : Bovine Lipoprotein lipase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa
      Observed band size: 55,60 kDa why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (45)

    Publishing research using ab21356? Please let us know so that we can cite the reference in this datasheet.

    ab21356 has been referenced in 45 publications.

    • Zhang M  et al. Erchen Decoction Mitigates Lipid Metabolism Disorder by the Regulation of PPAR? and LPL Gene in a High-Fat Diet C57BL/6 Mice Model. Evid Based Complement Alternat Med 2020:9102475 (2020). PubMed: 32256662
    • Kodama L  et al. Microglial microRNAs mediate sex-specific responses to tau pathology. Nat Neurosci 23:167-171 (2020). PubMed: 31873194
    • Zhou C  et al. Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis. Sci Adv 6:N/A (2020). PubMed: 32917715
    • Zheng X  et al. Selenoprotein F knockout leads to glucose and lipid metabolism disorders in mice. J Biol Inorg Chem 25:1009-1022 (2020). PubMed: 32995962
    • Yang H  et al. Fluoxetine regulates glucose and lipid metabolism via the PI3K-AKT signaling pathway in diabetic rats. Mol Med Rep 22:3073-3080 (2020). PubMed: 32945450
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 19 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Roche Blocking Reagent as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 11 2021

    Immunohistochemistry free floating abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain (PFA fixed))
    Specification
    Brain (PFA fixed)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 05 2020

    Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 11 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1.5µg/mL · Temperature: 23°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citra Plus
    Sample
    Mouse Tissue sections (Small Intestine)
    Specification
    Small Intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 28 2013

    Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    30 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Dongil Kim

    Verified customer

    Submitted Apr 11 2013

    Question

    Can you tell me if the epitope of this Ab is located in an extracellular, cytoplasmatic or transmembrane region of the LPL protein.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2013

    Answer

    This antibody was raised against the whole Human LPL whole protein as stated in the datasheet.

    Unfortunately, the exact epitope is unknown as the whole protein with all regions intact was used to produce the antibody and the epitope has not been mapped.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Oct 23 2013

    Question

    Yes, that’s my order. A credit would be greatly appreciated. The conc we used was 1:10,000.

    Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Jun 20 2012

    Answer

    Thank you for your reply and for confirming those details.

    Your credit note ID is ***.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting departmentat us.credits@abcam.com or by telephone at 888-772-2226. Please refer to the credit note ID in any correspondence with our accounting department.

    Please let me know if there is anything else that we can do for you and I'll be happy to help. Thank you again for your patience in resolving these issues.

    Read More

    Abcam Scientific Support

    Answered on Jun 20 2012

    Question

    Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

    The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

    Thanks so much for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 19 2012

    Answer

    One more thing. Could you please tell me what dilution you've tried with this secondary antibody, for our records?

    Thank you so much!

    Read More

    Abcam Scientific Support

    Answered on Jun 19 2012

    Question

    Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

    The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

    Thanks so much for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 19 2012

    Answer

    Thank you for your reply.

    I have recorded all of the information so that we can further investigate the trouble with the antibody. I again apologize that it is not working as expecting, but I'm glad you've found a way to get some results.

    With the ab98693, I've found an order on the PO#***. Can you confirm that this is your order? I will process the credit or refund, whichever you prefer.

    I look forward to hearing from you. Please let me know if you have any further questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Jun 19 2012

    Question

    I apologize to replying this late. We did more exp and found that we really need to use more antibody to “see” the bands on the membrane. So the best concentration for this antibody is 1:250 and overnight incubation at 4degC. Did you manage to retrieve the QC data for this antibody?

    As for the secondary, the abcam one actually did not work. Thanks again for your help

    Read More

    Abcam community

    Verified customer

    Asked on Jun 18 2012

    Answer

    Thank you for your reply and for keeping me updated.

    I am glad to hear that some bands were seen on the membrane, but I am sorry that it took such a high concentration in order to work in this assay. I have only been able to get the QC data for the first lot that you used, which is attached to this email. The left lane shows human LPL and the right lane shows bovine LPL. The QC data for the new lot is not available at this time.

    Which secondary antibody did not work? I will be happy to refund or replace this antibody if you would like.

    I look forward to hearing from you. Please let me know if you have further questions or if there is anything else that we can do.

    Read More

    Abcam Scientific Support

    Answered on Jun 18 2012

    1-10 of 19 Abreviews or Q&A

    •  Previous
    • 1
    • 2
    • Next 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.