Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)

Reviews (8)Q&A (11)References (44)
Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
  • Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)

Key features and details

  • Mouse monoclonal [LPL.A4] to Lipoprotein lipase
  • Suitable for: Flow Cyt, WB
  • Reacts with: Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Lipoprotein lipase antibody [LPL.A4]
    See all Lipoprotein lipase primary antibodies

  • Description

    Mouse monoclonal [LPL.A4] to Lipoprotein lipase

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Cow, Human

  • Immunogen

    Full length protein (Human).

  • Positive control

    • Purified human and bovine recombinant Lipoprotein lipase.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (4)
1/1000. Predicted molecular weight: 53 kDa.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 53 kDa.

Target

  • Function

    The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.

  • Involvement in disease

    Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.

  • Sequence similarities

    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.

  • Post-translational
    modifications

    Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

  • Cellular localization

    Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 3.1.1 antibody
      • EC 3.1.1.34 antibody
      • HDLCQ11 antibody
      • LIPD antibody
      • LIPL_HUMAN antibody
      • Lipoprotein lipase antibody
      • LPL antibody
      • LPL protein antibody
      • MGC137861 antibody
      see all

    Images

    • Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      All lanes : Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

      Lane 1 : Human Lipoprotein lipase.
      Lane 2 : Bovine Lipoprotein lipase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa
      Observed band size: 55,60 kDa why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References (44)

    Publishing research using ab21356? Please let us know so that we can cite the reference in this datasheet.

    ab21356 has been referenced in 44 publications.

    • Zhang M  et al. Erchen Decoction Mitigates Lipid Metabolism Disorder by the Regulation of PPAR? and LPL Gene in a High-Fat Diet C57BL/6 Mice Model. Evid Based Complement Alternat Med 2020:9102475 (2020). PubMed: 32256662
    • Li H  et al. Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis. Nutr Metab (Lond) 17:34 (2020). PubMed: 32377219
    • Kodama L  et al. Microglial microRNAs mediate sex-specific responses to tau pathology. Nat Neurosci 23:167-171 (2020). PubMed: 31873194
    • Zhou C  et al. Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis. Sci Adv 6:N/A (2020). PubMed: 32917715
    • Zheng X  et al. Selenoprotein F knockout leads to glucose and lipid metabolism disorders in mice. J Biol Inorg Chem 25:1009-1022 (2020). PubMed: 32995962
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 19 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Roche Blocking Reagent as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2021

    Immunohistochemistry free floating abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain (PFA fixed))
    Specification
    Brain (PFA fixed)
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 05 2020

    Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 11 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1.5µg/mL · Temperature: 23°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citra Plus
    Sample
    Mouse Tissue sections (Small Intestine)
    Specification
    Small Intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 28 2013

    Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    30 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Mr. Dongil Kim

    Verified customer

    Submitted Apr 11 2013

    Question

    Can you tell me if the epitope of this Ab is located in an extracellular, cytoplasmatic or transmembrane region of the LPL protein.

    Read More
    Answer

    This antibody was raised against the whole Human LPL whole protein as stated in the datasheet.

    Unfortunately, the exact epitope is unknown as the whole protein with all regions intact was used to produce the antibody and the epitope has not been mapped.

    Read More

    Question

    Yes, that’s my order. A credit would be greatly appreciated. The conc we used was 1:10,000.

    Thanks

    Read More
    Answer

    Thank you for your reply and for confirming those details.

    Your credit note ID is ***.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting departmentat us.credits@abcam.com or by telephone at 888-772-2226. Please refer to the credit note ID in any correspondence with our accounting department.

    Please let me know if there is anything else that we can do for you and I'll be happy to help. Thank you again for your patience in resolving these issues.

    Read More

    Question

    Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

    The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

    Thanks so much for your help.

    Read More
    Answer

    One more thing. Could you please tell me what dilution you've tried with this secondary antibody, for our records?

    Thank you so much!

    Read More

    Question

    Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

    The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

    Thanks so much for your help.

    Read More
    Answer

    Thank you for your reply.

    I have recorded all of the information so that we can further investigate the trouble with the antibody. I again apologize that it is not working as expecting, but I'm glad you've found a way to get some results.

    With the ab98693, I've found an order on the PO#***. Can you confirm that this is your order? I will process the credit or refund, whichever you prefer.

    I look forward to hearing from you. Please let me know if you have any further questions or if there is anything else that we can do for you.

    Read More

    Question

    I apologize to replying this late. We did more exp and found that we really need to use more antibody to “see” the bands on the membrane. So the best concentration for this antibody is 1:250 and overnight incubation at 4degC. Did you manage to retrieve the QC data for this antibody?

    As for the secondary, the abcam one actually did not work. Thanks again for your help

    Read More
    Answer

    Thank you for your reply and for keeping me updated.

    I am glad to hear that some bands were seen on the membrane, but I am sorry that it took such a high concentration in order to work in this assay. I have only been able to get the QC data for the first lot that you used, which is attached to this email. The left lane shows human LPL and the right lane shows bovine LPL. The QC data for the new lot is not available at this time.

    Which secondary antibody did not work? I will be happy to refund or replace this antibody if you would like.

    I look forward to hearing from you. Please let me know if you have further questions or if there is anything else that we can do.

    Read More

    1-10 of 19 Abreviews or Q&A

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