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    lipt2-antibody-ab122641.pdf

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Signal Transduction Metabolism Lipid metabolism
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Anti-LIPT2 antibody (ab122641)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-LIPT2 antibody (ab122641)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPT2 antibody (ab122641)

Key features and details

  • Rabbit polyclonal to LIPT2
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-LIPT2 antibody
  • Description

    Rabbit polyclonal to LIPT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: CAIGVRCGRHITSHGLALNCSTDLTWFEHIVPCGLVGTGVTSLSKELQRH VTVEEVMPPFLVA FKEIYKCTLISEDS, corresponding to amino acids 153-229 of Human LIPT2. (A6NK58).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human rectum tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Lipid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab122641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Target

  • Function

    Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
  • Pathway

    Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.
  • Sequence similarities

    Belongs to the lipB family.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession A6NK58 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 387787 Human
    • SwissProt: A6NK58 Human
    • Unigene: 591971 Human
    • Alternative names

      • EC 2.3.1.181 antibody
      • FCT2 antibody
      • Lipoate-protein ligase B antibody
      • Lipoyl(octanoyl) transferase 2 (putative) antibody
      • Lipoyl/octanoyl transferase antibody
      • Lipt2 antibody
      • LIPT2_HUMAN antibody
      • mitochondrial antibody
      • Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase antibody
      • OTTHUMP00000230589 antibody
      • OTTHUMP00000230590 antibody
      • OTTHUMP00000230591 antibody
      • Putative lipoyltransferase 2 antibody
      • Putative lipoyltransferase 2, mitochondrial antibody
      • Putative octanoyltransferase, mitochondrial antibody
      • SLC22A16 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-LIPT2 antibody (ab122641)
      Immunocytochemistry/ Immunofluorescence - Anti-LIPT2 antibody (ab122641)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122641 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPT2 antibody (ab122641)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPT2 antibody (ab122641)
      ab122641, at 1/20, staining LIPT2 in paraffin embedded Human rectum by Immunohistochemistry.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab122641? Please let us know so that we can cite the reference in this datasheet.

    ab122641 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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