Anti-LIPT2 antibody (ab122641)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LIPT2 antibody
See all LIPT2 primary antibodies -
Description
Rabbit polyclonal to LIPT2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: CAIGVRCGRHITSHGLALNCSTDLTWFEHIVPCGLVGTGVTSLSKELQRH VTVEEVMPPFLVA FKEIYKCTLISEDS, corresponding to amino acids 153-229 of Human LIPT2. (A6NK58).
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Positive control
- Human rectum tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Function
Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate. -
Pathway
Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2. -
Sequence similarities
Belongs to the lipB family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 387787 Human
- SwissProt: A6NK58 Human
- Unigene: 591971 Human
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Alternative names
- EC 2.3.1.181 antibody
- FCT2 antibody
- Lipoate-protein ligase B antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LIPT2 antibody (ab122641)Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122641 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPT2 antibody (ab122641)ab122641, at 1/20, staining LIPT2 in paraffin embedded Human rectum by Immunohistochemistry.
Datasheets and documents
References
ab122641 has not yet been referenced specifically in any publications.
