Overview

  • Product name
    Anti-LIPT2 antibody
    See all LIPT2 primary antibodies
  • Description
    Rabbit polyclonal to LIPT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: CAIGVRCGRHITSHGLALNCSTDLTWFEHIVPCGLVGTGVTSLSKELQRH VTVEEVMPPFLVA FKEIYKCTLISEDS, corresponding to amino acids 153-229 of Human LIPT2. (A6NK58).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human rectum tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
  • Pathway
    Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.
  • Sequence similarities
    Belongs to the lipB family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
    • Alternative names
      • EC 2.3.1.181 antibody
      • FCT2 antibody
      • Lipoate-protein ligase B antibody
      • Lipoyl(octanoyl) transferase 2 (putative) antibody
      • Lipoyl/octanoyl transferase antibody
      • Lipt2 antibody
      • LIPT2_HUMAN antibody
      • mitochondrial antibody
      • Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase antibody
      • OTTHUMP00000230589 antibody
      • OTTHUMP00000230590 antibody
      • OTTHUMP00000230591 antibody
      • Putative lipoyltransferase 2 antibody
      • Putative lipoyltransferase 2, mitochondrial antibody
      • Putative octanoyltransferase, mitochondrial antibody
      • SLC22A16 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-LIPT2 antibody (ab122641)
      Immunocytochemistry/ Immunofluorescence - Anti-LIPT2 antibody (ab122641)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122641 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPT2 antibody (ab122641)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPT2 antibody (ab122641)
      ab122641, at 1/20, staining LIPT2 in paraffin embedded Human rectum by Immunohistochemistry.

    References

    ab122641 has not yet been referenced specifically in any publications.

    Publishing research using ab122641? Please let us know so that we can cite the reference in this datasheet.

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