• Product name
  • Description
    Goat polyclonal to LIS1
  • Host species
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Horse, Chicken, Cow, Cat, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 397-410 of Human LIS1 (NP_000421)

  • Positive control
    • Human pancreas, human thyroid and human brain, cortex tissues


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab117457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
  • Tissue specificity
    Fairly ubiquitous expression in both the frontal and occipital areas of the brain.
  • Involvement in disease
    Defects in PAFAH1B1 are the cause of lissencephaly type 1 (LIS1) [MIM:607432]; also known as classic lissencephaly. LIS1 is characterized by agyria or pachgyria and disorganization of the clear neuronal lamination of normal six-layered cortex. The cortex is abnormally thick and poorly organized with 4 primitive layers. LIS1 is associated with enlarged and dysmorphic ventricles and often hypoplasia of the corpus callosum.
    Defects in PAFAH1B1 are the cause of subcortical band heterotopia (SBH) [MIM:607432]. SBH is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface.
    Defects in PAFAH1B1 are a cause of Miller-Dieker lissencephaly syndrome (MDLS) [MIM:247200]. MDLS is a contiguous gene deletion syndrome of chromosome 17p13.3, characterized by classical lissencephaly and distinct facial features. Additional congenital malformations can be part of the condition.
  • Sequence similarities
    Belongs to the WD repeat LIS1/nudF family.
    Contains 1 LisH domain.
    Contains 7 WD repeats.
  • Domain
    Dimerization mediated by the LisH domain may be required to activate dynein.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Nucleus membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIS 1 antibody
    • LIS 2 antibody
    • LIS-1 antibody
    • LIS1 antibody
    • LIS1_HUMAN antibody
    • LIS2 antibody
    • Lissencephaly 1 protein antibody
    • Lissencephaly-1 protein antibody
    • MDCR antibody
    • MDS antibody
    • PAF acetylhydrolase 45 kDa subunit antibody
    • PAF AH 45 kDa subunit antibody
    • PAF AH alpha antibody
    • PAF-AH 45 kDa subunit antibody
    • PAF-AH alpha antibody
    • PAFAH alpha antibody
    • PAFAH antibody
    • PAFAH1B1 antibody
    • PAFAHA antibody
    • Platelet activating factor acetylhydrolase 1b regulatory subunit 1 antibody
    • Platelet activating factor acetylhydrolase isoform Ib alpha subunit antibody
    • Platelet-activating factor acetylhydrolase IB subunit alpha antibody
    see all


  • ab117457, at 3.75 µg/ml, staining LIS1 in formalin fixed, paraffin embedded Human pancreas tissue by Immunohistochemistry using a biotinylated anti goat IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
  • ab117457, at 3.75 µg/ml, staining LIS1 in formalin fixed, paraffin embedded Human thyroid tissue by Immunohistochemistry using a biotinylated anti goat IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
  • ab117457, at 3.75 µg/ml, staining LIS1 in formalin fixed, paraffin embedded Human brain cortes tissue by Immunohistochemistry using a biotinylated anti goat IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
  • Anti-LIS1 antibody (ab117457) at 0.1 µg/ml + Rat Ovary Lysates

    Predicted band size: 47 kDa


ab117457 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Here are the answers to your questionnaire and the picture of the WB.

1) Abcam product code ab117457

2) Abcam order reference number or product batch number: GR79418-1

3) Description of the problem: lower than expected MW

4) Sample preparation:

Type of sample: whole cell lysate of human keratinocytes

Lysis buffer: 50 mm Tris-HCl, pH 7.4, 1% Triton X-100, 150 mm NaCl, 5 mm EDTA, 0.2 mm sodium orthovanadate,

Protease inhibitors: yes (Complete Roche)

Phosphatase inhibitors: yes

Reducing agent: SDS, B-mercaptoethanol

Boiling for ≥5 min? yes, 10 minutes

Protein loaded ug/lane: 20ug

Positive control: none

Negative control: none

5) Percentage of gel: 10%

Type of membrane: nitrocellulose

Protein transfer verified: yes PonceauS red

Blocking agent and concentration: TBST 0,1%, 5% milk

Blocking time: 30 min

Blocking temperature: Room temp.

6) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution: 1:600

Diluent buffer: TBST 0,1%5% milk

Incubation time: 45 min

Incubation temperature: room temp

7) Secondary antibody:

Species: bovine

Reacts against: goat

Concentration or dilution: 1:1000

Diluent buffer: TBST 0,1% 5% milk

Incubation time: 30 minutes

Incubation temperature: Room Temp.

Fluorochrome or enzyme conjugate: Horse radish peroxydase

8) Washing after primary and secondary antibodies: yes

Buffer: TBST 0,1%

Number of washes: 6

9)Detection method:Chemiluminesence, Supersignal

10) How many times have you run this staining? 3

Do you obtain the same results every time? no (others WB: bands between 72 and 95 kDa)

What steps have you altered to try and optimize the use of this antibody? Different membranes with different cell lysates.

Thank you very much for your help.

Read More

Thank youfor your reply and for including the details of your experiment.

The predicted MW for the human protein is 46.6 kDa, according to SwissProt.


It looks like the band you're seeing is ˜10 kDa less.We see a band around 40 kDa in our testing (please see attached).I do think that you are seeing the protein of interest, and since the protein hastwo isoforms it may bea reason why theprotein is running a bit lower.The next best step to ensure the specificitywould be to run a positive control (we recommend rat ovary or human lung or ovary). Or, you could do a blocking experiment using the blocking peptide ab22930 to ensure the antibody's specificity.


I hope this information helps. Please contact us with any other questions.

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