Anti-LIS1 antibody (ab2607)

Reviews (2)Q&A (5)References (13)

Overview

  • Product name
    Anti-LIS1 antibody
    See all LIS1 primary antibodies
  • Description
    Rabbit polyclonal to LIS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human LIS1. Represents a portion of human Lissencephaly 1 (Lis 1) protein encoded in part by exons 3 and 4.

  • Positive control
    • TF-1 (erythroleukemia cell line) cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purification notes
    Affinity purified using the immunising peptideimmobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
IP
  • Application notes
    ICC/IF: Use at an assay dependent dilution (see PMID: 17314247).
    IP: Use at a concentration of 2 - 10 µg/ml.
    WB: 1/300 - 1/3000. Suggested working dilution of 1/1000 (see PMID: 17314247).
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
    • Tissue specificity
      Fairly ubiquitous expression in both the frontal and occipital areas of the brain.
    • Involvement in disease
      Defects in PAFAH1B1 are the cause of lissencephaly type 1 (LIS1) [MIM:607432]; also known as classic lissencephaly. LIS1 is characterized by agyria or pachgyria and disorganization of the clear neuronal lamination of normal six-layered cortex. The cortex is abnormally thick and poorly organized with 4 primitive layers. LIS1 is associated with enlarged and dysmorphic ventricles and often hypoplasia of the corpus callosum.
      Defects in PAFAH1B1 are the cause of subcortical band heterotopia (SBH) [MIM:607432]. SBH is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface.
      Defects in PAFAH1B1 are a cause of Miller-Dieker lissencephaly syndrome (MDLS) [MIM:247200]. MDLS is a contiguous gene deletion syndrome of chromosome 17p13.3, characterized by classical lissencephaly and distinct facial features. Additional congenital malformations can be part of the condition.
    • Sequence similarities
      Belongs to the WD repeat LIS1/nudF family.
      Contains 1 LisH domain.
      Contains 7 WD repeats.
    • Domain
      Dimerization mediated by the LisH domain may be required to activate dynein.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Nucleus membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • LIS 1 antibody
      • LIS 2 antibody
      • LIS-1 antibody
      • LIS1 antibody
      • LIS1_HUMAN antibody
      • LIS2 antibody
      • Lissencephaly 1 protein antibody
      • Lissencephaly-1 protein antibody
      • MDCR antibody
      • MDS antibody
      • PAF acetylhydrolase 45 kDa subunit antibody
      • PAF AH 45 kDa subunit antibody
      • PAF AH alpha antibody
      • PAF-AH 45 kDa subunit antibody
      • PAF-AH alpha antibody
      • PAFAH alpha antibody
      • PAFAH antibody
      • PAFAH1B1 antibody
      • PAFAHA antibody
      • Platelet activating factor acetylhydrolase 1b regulatory subunit 1 antibody
      • Platelet activating factor acetylhydrolase isoform Ib alpha subunit antibody
      • Platelet-activating factor acetylhydrolase IB subunit alpha antibody
      see all

    Images

    • Western blot - Anti-LIS1 antibody (ab2607)
      Western blot - Anti-LIS1 antibody (ab2607)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIS1 antibody (ab2607)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIS1 antibody (ab2607)
      ab2607 (2µg/ml) staining Lis1 in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic compartment of the mature spermatocyte cells.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    References

    This product has been referenced in:
    • Brehar FM  et al. Preferential Association of Lissencephaly-1 Gene Expression with CD133+ Glioblastoma Cells. J Cancer 8:1284-1291 (2017). IHC-P ; Human . Read more (PubMed: 28607604) »
    • Sudarov A  et al. Lis1 controls dynamics of neuronal filopodia and spines to impact synaptogenesis and social behaviour. EMBO Mol Med 5:591-607 (2013). IHC-P ; Mouse . Read more (PubMed: 23483716) »
    See all 13 Publications for this product

    Publishing research using ab2607? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LIS1 antibody

     Poor
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Embryo)
    Permeabilization
    Yes - 0,5%TritonX + 0,05%Tween20
    Specification
    Embryo
    Blocking step
    Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Lynn Vandenberghe

    Verified customer

    Submitted Aug 17 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LIS1 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Caco-2 cell)
    Specification
    Caco-2 cell
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 28 2013

    Question

    Thank you very much for your email. If we would accept your offer of a free charge of replacement, will you send another sample of the same Anti-Lis1 antibody with the same Lot number, or can you send us a new sample with the old lot number which was working in our experiments?
    Secondly, I have checked your website for several other Anti-Lis1 antibodies which we can use in Western Blot and also in Immunohistochemistry. I have found two different Anti-Lis1 antibodies with the code numbers ab115357 and ab117457. But actually, in the website there were no images regarding their Western blot performance. Could you please send me some Western blot images of these antibodies if you have, or some reference articles where I can see some Western blot results regarding these antibodies?
    Furthermore, I would appreciate when you could advice additional Anti-Lis1 antibodies.
    Thank you in advance for your help and interest,

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    We will not send you a sample of either a new or old lot of the ab2607 as we are not able to provide samples with over 100.000 products, but we will gladly send you a whole vial of this product, which is fully covered by our gurantee as well. So if the problem should persist, please contact us again so we can discuss on how to proceed.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 123456.

    To check the status of the order please contact our Customer Service team and reference this number.


    In addition, I will contact the lab to enquire about a WB image for the two other antibodies asd requested.

    I hope this is helpful to you. Please do not hesitate to contact us again if you have more question or need more information.

    Best regards,

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    Question

    It will be neither paraffin or fresh frozen. We perform whole-mount staining following fixation.

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    Answer

    Thank you for your reply.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab2607 in IHC-whole mount. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-whole mount and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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    Question

    I would like to test the following product for IF on PFA fixed mouse inner ear tissues:

    Anti-LIS1 antibody (ab2607)

    Read More
    Answer

    Thank you for contacting us.

    Will the mouse tissue be paraffin embedded or fresh frozen, or neither of them?

    Could you please let me know? Thank you.


    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE HeLa cell lysate PRIMARY ANTIBODY ab2607 anti-Lis1 at 1:1000 in 5% Milk in TBST for 1 hour DETECTION METHOD ECLPlus POSITIVE AND NEGATIVE CONTROLS USED Used HeLa lysate transfected with different genes, negative control is eGFP transfection, and no positive control ANTIBODY STORAGE CONDITIONS 4degC SAMPLE PREPARATION Spin down HeLa cells in DMEM, wash once with 1xPBS + 1mM EDTA on ice, spin down cells at 6,000 rpm for 2 min, remove supernatant, add 100ul 2x SDS sample buffer to pellet and boil 3-5 min AMOUNT OF PROTEIN LOADED 10% of SDS sample buffer ELECTROPHORESIS/GEL CONDITIONS 15% PAGE TRANSFER AND BLOCKING CONDITIONS Block over night in 5% Milk in TBST SECONDARY ANTIBODY anti-Rabbit HRP(GE Healthcare NA934V) 1:5000 in 5% Milk in TBST for 40 min HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Also tried non-transfected cells

    Read More
    Answer

    Thank you for contacting Abcam. I have looked over all the details that you provided and I would recommended that you increase you incubation of the primary antibody to an overnight incubation at 4C with gently agitation. If increasing the incubation time does not prove to be effective then please let me know. The antibody is covered under our Abpromise and so is guaranteed to work in western blot on human and mouse samples. If we cannot resolve this issue for you, then I would be happy. If there is anything else I can help with, please let me know.

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    Question

    DESCRIPTION OF THE PROBLEM The previous batch of antibody worked perfectly on WB(unfortunately I don't know the Lot number) using regular protocol: 25mkg of total cell lysate, 3% milk blocking etc. The next batch of the same antibody (Lot 38079) which I got is not working at all using the same WB conditions. It gives 1 non-specific band of the wrong molecular weight. SAMPLE HeLa DETECTION METHOD ECL ANTIBODY STORAGE CONDITIONS +4C AMOUNT OF PROTEIN LOADED 25mkg HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

    Read More
    Answer

    Thank you for your patience; I was away on vacation and could not reply to you earlier. As you previously used a different lot of ab2607 successfully under the same conditions, my hunch is that the latest vial that you received somehow went off. Please send me your shipping address including phone number and I will send you another vial free of charge.

    Read More

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