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DESCRIPTION OF THE PROBLEM The previous batch of antibody worked perfectly on WB(unfortunately I don't know the Lot number) using regular protocol: 25mkg of total cell lysate, 3% milk blocking etc. The next batch of the same antibody (Lot 38079) which I got is not working at all using the same WB conditions. It gives 1 non-specific band of the wrong molecular weight. SAMPLE HeLa DETECTION METHOD ECL ANTIBODY STORAGE CONDITIONS +4C AMOUNT OF PROTEIN LOADED 25mkg HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
Asked on Mar 15 2004
Thank you for your patience; I was away on vacation and could not reply to you earlier. As you previously used a different lot of ab2607 successfully under the same conditions, my hunch is that the latest vial that you received somehow went off. Please send me your shipping address including phone number and I will send you another vial free of charge.
Answered on Mar 24 2004