Immunocytochemistry/ Immunofluorescence abreview for Anti-LIS1 antibody

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Embryo)
Permeabilization
Yes - 0,5%TritonX + 0,05%Tween20
Specification
Embryo
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 10% goat serum + 0,05 Tween20
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Abcam response
Thank you very much for submitting an AbReview. The antibody has been used to stain whole mount sections (IHC-whole mount), an application that has not been tested before. We appreciate the time and effort that the reviewer has put in to test testing. It may be possible that the antibody is not suitable for this application.

Lynn Vandenberghe

Verified customer

Submitted Aug 17 2016

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