Overview

  • Product name
    Anti-Listeria monocytogenes antibody
    See all Listeria monocytogenes primary antibodies
  • Description
    Rabbit polyclonal to Listeria monocytogenes
  • Host species
    Rabbit
  • Specificity
    Recognizes whole cells of Listeria monocytogenes.
  • Tested applications
    Suitable for: Conjugation, ELISAmore details
  • Species reactivity
    Reacts with Group A Streptococcus, Group B Streptococcus, S. pneumoniae, S. aureus, Clostridium perfringens and Bacillus subtilis. Not yet tested in other species.
  • Immunogen

    Cell preparation: Listeria monocytogenes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
  • Alternative names
    • L. monocytogenes antibody

References

ab20506 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest.

I can confirm that this antibody has only been tested for Agglutination - but not for IP (immunoprecipitaion) or conjugation. Alternatively, you may be interested in ab20506 which has been characterized for conjugation and ELISA. The price and unit size can be viewed on the on-line product datasheet once you select the home country.

The shipping, handling & packaging charges can be found on our website as well:

https://www.abcam.com/index.html?pageconfig=help#shippingcharges

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

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Answer

Thank you for contacting us. The Listeria monocytogenes antibody ab20506 has been validated for conjugation to biotin, FITC, and HRP. We have not conjugated it to magnetic beads in house, but it should be suitable for that purpose. Our protocol for coupling antibodies to Protein A or G beads can be found on the following site: https://www.abcam.com/index.html?pageconfig=resource&rid=11415 For magnetic beads, I would recommend following the protocol provided by the manufacturer of the beads. I hope this helps, please let me know if you need any additional information.

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Answer

Thank you for your enquiry. The immunogen used for Rabbit anti Listeria monocytogenes ab20506 is a mixture of intact and disrupted L. monocytogenes organisms. We have not tested this antibody for reactivity with heat-killed L. monocytogenes cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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