Anti-Listeria monocytogenes antibody (ab35132)
Key features and details
- Rabbit polyclonal to Listeria monocytogenes
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Listeria monocytogenes
- Isotype: IgG
Overview
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Product name
Anti-Listeria monocytogenes antibody
See all Listeria monocytogenes primary antibodies -
Description
Rabbit polyclonal to Listeria monocytogenes -
Host species
Rabbit -
Specificity
This antibody is specific for Listeria monocytogenes whole cells. This antiserum has not been absorbed and may react with related microorganisms.
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Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Listeria monocytogenes -
Immunogen
Tissue, cells or virus corresponding to Listeria monocytogenes. Listeria monocytogenes. whole cells. ATCC strain 43251.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purification notes
>95% pure. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
ELISA | ||
IHC-P | ||
ICC/IF |
Notes |
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ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. (Pre-treatment with 0.1% pronase was used)
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection. -
Database links
- Entrez Gene: 2831193 Listeria monocytogenes
- SwissProt: C1L1U9 Listeria monocytogenes
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Alternative names
- L. monocytogenes antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab35132 has been referenced in 17 publications.
- Zhou Z et al. A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognition. Anal Chim Acta 1160:338421 (2021). PubMed: 33894956
- Cho K et al. Listeria monocytogenes Establishes Commensalism in Germ-Free Mice Through the Reversible Downregulation of Virulence Gene Expression. Front Immunol 12:666088 (2021). PubMed: 34012449
- Volmari A et al. Leukocyte-Derived High-Mobility Group Box 1 Governs Hepatic Immune Responses to Listeria monocytogenes. Hepatol Commun 5:2104-2120 (2021). PubMed: 34558858
- Sai K et al. Necroptosis mediators RIPK3 and MLKL suppress intracellular Listeria replication independently of host cell killing. J Cell Biol 218:1994-2005 (2019). PubMed: 30975711
- Costa AC et al. Stathmin recruits tubulin to Listeria monocytogenes-induced actin comets and promotes bacterial dissemination. Cell Mol Life Sci N/A:N/A (2018). PubMed: 30506415