Overview

  • Product name
    Anti-Listeria monocytogenes antibody (Biotin)
    See all Listeria monocytogenes primary antibodies
  • Description
    Rabbit polyclonal to Listeria monocytogenes (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cyt, ELISA, IHC-Frmore details
  • Species reactivity
    Antiserum is not absorbed and may react with other related microorganisms. Reacts with Group A Streptococcus, Group B Streptococcus, S. pneumoniae, Staph aureus, clostridium perfingens and Bacillus subtilis. Not yet tested in other species.
  • Immunogen

    Tissue, cells or virus corresponding to Listeria monocytogenes Listeria monocytogenes.

  • General notes

    IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ELISA 1/1000.
IHC-Fr 1/250.

Target

  • Relevance
    The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
  • Alternative names
    • L. monocytogenes antibody

Images

  • ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1:20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1:500 in PBS for 30 min at 20°C.

    See Abreview

  • Flow cytometry analysis of Listeria monocytogenes in culture, staining Listeria monocytogenes with ab20766.

    The sample was incubated with the primary antibody (1/300 in PBS) for 30 minutes at 4°C. A Streptavidin-Peridinin Chlorophyll-a Protein conjugated with PerCP was used for detection.

    Gating Strategy: FSC/SSC

    See Abreview

References

This product has been referenced in:
  • Dresing P  et al. A fluorescence reporter model defines "Tip-DCs" as the cellular source of interferon ß in murine listeriosis. PLoS One 5:e15567 (2010). IHC-Fr . Read more (PubMed: 21179567) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Flow Cytometry
Sample
Listeria monocytogenes Cell (Listeria monocytogenes in culture)
Specification
Listeria monocytogenes in culture
Permeabilization
No
Gating Strategy
FSC/SSC

Abcam user community

Verified customer

Submitted Nov 22 2012

Question
Answer

DISCOUNT CODE: 100%ABR-00000

Ablaufdatum: 20. November 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab20766 in FACS zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 100%ABR-00000, deckt den Wert von ab20766 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test inFACS eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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Answer

Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1:20 Cd16/32, normal rat normal mouse as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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