Key features and details
- Rabbit polyclonal to Listeria monocytogenes (Biotin)
- Suitable for: Flow Cyt, ELISA, IHC-Fr
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Listeria monocytogenes antibody (Biotin)
See all Listeria monocytogenes primary antibodies
DescriptionRabbit polyclonal to Listeria monocytogenes (Biotin)
Tested applicationsSuitable for: Flow Cyt, ELISA, IHC-Frmore details
Species reactivityReacts with: Other species
Tissue, cells or virus corresponding to Listeria monocytogenes Listeria monocytogenes.
IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab20766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
- L. monocytogenes antibody
ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1:20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1:500 in PBS for 30 min at 20°C.
Flow cytometry analysis of Listeria monocytogenes in culture, staining Listeria monocytogenes with ab20766.
The sample was incubated with the primary antibody (1/300 in PBS) for 30 minutes at 4°C. A Streptavidin-Peridinin Chlorophyll-a Protein conjugated with PerCP was used for detection.
Gating Strategy: FSC/SSC
ab20766 has been referenced in 1 publication.
- Dresing P et al. A fluorescence reporter model defines "Tip-DCs" as the cellular source of interferon ß in murine listeriosis. PLoS One 5:e15567 (2010). IHC-Fr . PubMed: 21179567