Overview

  • Product name

    Anti-Listeria monocytogenes antibody (Biotin)
    See all Listeria monocytogenes primary antibodies
  • Description

    Rabbit polyclonal to Listeria monocytogenes (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Flow Cyt, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue, cells or virus corresponding to Listeria monocytogenes Listeria monocytogenes.

  • General notes

    IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab20766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ELISA 1/1000.
IHC-Fr 1/250.

Target

  • Relevance

    The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
  • Alternative names

    • L. monocytogenes antibody

Images

  • ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1:20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1:500 in PBS for 30 min at 20°C.

    See Abreview

  • Flow cytometry analysis of Listeria monocytogenes in culture, staining Listeria monocytogenes with ab20766.

    The sample was incubated with the primary antibody (1/300 in PBS) for 30 minutes at 4°C. A Streptavidin-Peridinin Chlorophyll-a Protein conjugated with PerCP was used for detection.

    Gating Strategy: FSC/SSC

    See Abreview

References

This product has been referenced in:

  • Dresing P  et al. A fluorescence reporter model defines "Tip-DCs" as the cellular source of interferon ß in murine listeriosis. PLoS One 5:e15567 (2010). IHC-Fr . Read more (PubMed: 21179567) »
See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

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1-2 of 2 Abreviews

Application
Flow Cytometry
Sample
Listeria monocytogenes Cell (Listeria monocytogenes in culture)
Specification
Listeria monocytogenes in culture
Permeabilization
No
Gating Strategy
FSC/SSC

Abcam user community

Verified customer

Submitted Nov 22 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1:20 Cd16/32, normal rat normal mouse as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 17 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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