Immunohistochemistry (Frozen sections) abreview for Anti-Listeria monocytogenes antibody (Biotin)

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Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1:20 Cd16/32, normal rat normal mouse as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Other product details

Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
Spleen fixed in 4% PFA for 2h @ RT, washed 2x in PBS, then incubated ON in 30% Sucrose in PBS @ 4°C, froze away in OCT/Tissuetek.
In general we use Tyramide Signal amplification for generate a brighter staining (e.g. PerkinElmer) but here not used. Detection with Streptavidin-Cy3 (Caltag, 1:500 in PBS for 30 min @ 20°C).
The attatched picture shows a spleen section from a mice infected with 1x10exp6 L. monocytogenes for 24h. Nuclei were stained with DAPI shown in grey.

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Submitted Jul 17 2009

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