Overview

  • Product name

    Anti-Listeriolysin (LLO) antibody
  • Description

    Rabbit polyclonal to Listeriolysin (LLO)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Listeria monocytogenes
  • Immunogen

    Recombinant full length protein corresponding to Listeriolysin (LLO). (Expressed in E.coli).

  • General notes

    Listeriolysin O (LLO), a major virulence factor produced by all pathogenic strains of
    L. monocytogenes, has been identified as a candidate antigen for a serological assay.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 2.94% Sodium citrate, 0.61% Tris, 0.05% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1500 - 1/3000.
WB 1/1000 - 1/1500. Detects a band of approximately 56 kDa (predicted molecular weight: 59 kDa).

Target

  • Relevance

    Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.
  • Database links

    • Alternative names

      • hly antibody
      • hlyA antibody
      • lisA antibody
      • LLO antibody
      • Thiol activated cytolysin antibody
      see all

    References

    This product has been referenced in:

    • Maurer J  et al. Missing elimination via membrane vesicle shedding contributes to the diminished calcium sensitivity of listeriolysin O. Sci Rep 8:15846 (2018). Read more (PubMed: 30367146) »
    • Salerno-Gonçalves R  et al. Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. PLoS Negl Trop Dis 11:e0005912 (2017). WB . Read more (PubMed: 28873442) »
    See all 9 Publications for this product

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