Key features and details
- Rabbit polyclonal to Listeriolysin (LLO)
- Suitable for: ELISA, WB
- Reacts with: Listeria monocytogenes
- Isotype: IgG
Product nameAnti-Listeriolysin (LLO) antibody
DescriptionRabbit polyclonal to Listeriolysin (LLO)
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Listeria monocytogenes
Recombinant full length protein corresponding to Listeriolysin (LLO). (Expressed in E.coli).
Listeriolysin O (LLO), a major virulence factor produced by all pathogenic strains of
L. monocytogenes, has been identified as a candidate antigen for a serological assay.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 2.94% Sodium citrate, 0.61% Tris, 0.05% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1500 - 1/3000.|
|WB||1/1000 - 1/1500. Detects a band of approximately 56 kDa (predicted molecular weight: 59 kDa).|
RelevanceListeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.
- hly antibody
- hlyA antibody
- lisA antibody
ab200538 has been referenced in 10 publications.
- Coelho C et al. Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles. J Biol Chem 294:1202-1217 (2019). PubMed: 30504226
- Maurer J et al. Missing elimination via membrane vesicle shedding contributes to the diminished calcium sensitivity of listeriolysin O. Sci Rep 8:15846 (2018). PubMed: 30367146
- Salerno-Gonçalves R et al. Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. PLoS Negl Trop Dis 11:e0005912 (2017). WB . PubMed: 28873442
- Stachowiak R et al. Cytotoxicity of purified listeriolysin O on mouse and human leukocytes and leukaemia cells. BMC Biotechnol 14:77 (2014). WB . PubMed: 25134983
- Arnett E et al. The pore-forming toxin listeriolysin O is degraded by neutrophil metalloproteinase-8 and fails to mediate Listeria monocytogenes intracellular survival in neutrophils. J Immunol 192:234-44 (2014). WB . PubMed: 24319266
- Burg-Golani T et al. Membrane chaperone SecDF plays a role in the secretion of Listeria monocytogenes major virulence factors. J Bacteriol 195:5262-72 (2013). Listeria monocytogenes . PubMed: 24056100
- Lee JH et al. Transcription factor sB plays an important role in the production of extracellular membrane-derived vesicles in Listeria monocytogenes. PLoS One 8:e73196 (2013). WB ; Listeria monocytogenes . PubMed: 23977379
- Vadia S et al. The pore-forming toxin listeriolysin O mediates a novel entry pathway of L. monocytogenes into human hepatocytes. PLoS Pathog 7:e1002356 (2011). WB ; Listeria monocytogenes . PubMed: 22072970
- Lehrer RI et al. Human alpha-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins. Infect Immun 77:4028-40 (2009). PubMed: 19581399
- Giese B et al. Staphylococcal alpha-toxin is not sufficient to mediate escape from phagolysosomes in upper-airway epithelial cells. Infect Immun 77:3611-25 (2009). PubMed: 19564384