Overview

  • Product name

    Lithium Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Other biological fluids
  • Assay type

    Quantitative
  • Sensitivity

    100 µM
  • Species reactivity

    Reacts with: Human
  • Product overview

    Lithium Assay Kit (Colorimetric) (ab235613) is designed for the quantification of lithium levels in biological fluids such as serum and plasma.


    The assay uses a lithium-selective bi-chromatic probe that undergoes an absorbance change at two distinct wavelengths upon binding to Li+1 = 540 nm, λ2 = 630 nm). The ratio of the two optical measurements is used to accurately calculate sample Li+ concentration. The kit also includes a sodium masking reagent to prevent the possibility of interference by supra-physiological levels of serum Na+ (hypernatremia). Endogenous mono-, di-, and trivalent ions do not interfere with the assay.


    The assay is quick, is high-throughput adaptable and has a linear range from 0.5 – 10 nmole lithium per well (corresponding to 0.1 – 2 mM serum lithium). The kit can detect lithium concentration as low as 100 µM in human serum.


     

  • Notes

    Lithium, the lightest alkali metal element, is a ductile, reactive metal that occurs in nature as the Li+ cation and is found in various mineral compounds. Physiologically, lithium is present only in trace levels and is not considered to be an essential dietary nutrient. However, lithium is routinely used in medicine as psychoactive drug. Lithium acts as a mood stabilized and is considered to be the gold standard first-line treatment for bipolar depression and acute mania. Patients treated with lithium are less likely to require hospitalization or complete suicide than patients treated with other mood stabilizers or antipsychotics. Chemically, lithium is the simplest possible drug - as an element, it is not metabolized by the body and has zero-order renal elimination kinetics. Lithium has a narrow therapeutic/toxic ratio and requires blood level monitoring, both to ensure efficacy and decrease the risk of toxic side-effects. The target serum concentration for lithium maintenance therapy ranges from 0.6-1.2 mM. At serum levels 1.5 mM there is a sharp increase in severe adverse effects. Overt, potentially fatal side effects (such as seizures, ataxia and loss of consciousness) are observed at serum levels > 2.5 mM.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Lithium Assay Buffer 1 x 25ml
    Lithium Standard (10 mM) 1 x 500µl
    Probe Solution 1 x 10ml
    Sodium-Masking Solution 1 x 1.5ml

Images

  • Lithium concentration is directly proportional to the ratio of absorbance measured at 540 nm and 630 nm.

  • All other cations tested contribute ≤10% interference when normalized to the signal generated by 10 nmole lithium.

  • Mean lithium concentrations detected in the spiked samples were 0.52 mM, 1.03 mM, 1.53 mM and 2.01 mM, respectively (mean spike recovery rates across all spiked concentrations ranged from 100.3 – 105.5%). Potential interference by excessive serum sodium (hypernatremia) was also tested. Normal human serum (5 μl, undiluted) was spiked with an additional 40 mM NaCl to simulate hypernatremic conditions (serum Na+ ≥175 mM). The signal imparted by the additional 40 mM Na was equivalent to that of 0.062 mM lithium (≤10% interference for the typical therapeutic range). Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.

Protocols

References

ab235613 has not yet been referenced specifically in any publications.

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