• Product name
    Live and Dead Cell Assay
  • Detection method
  • Product overview

    ab115347 is a one-step assay to differentially labels live and dead cells with fluorescent dyes. It is useful for the rapid quantitation of cell viability using flow cytometry or fluorescent microscopy. The provided Live and Dead Assay stain is sufficient for ~1000 assays.


    The Live and Dead assay stain solution is a mixture of two highly fluorescent dyes that differentially label live and dead cells:


    The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).


    The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm, respectively.


    The Live and Dead Cell Assay is a one-step staining procedure that is simple and fast. It can be used directly in cell culture media. Stained cells are not compatible with fixation.


  • Notes

    Store the Live and Dead staining solution at -20°C. Allow the product to warm to room temperature before opening. Use diluted solutions of Live/Dead stain promptly.

  • Platform



  • Dot plots showing live/dead analysis of vehicle or drug treated Jurkat cells (day 3 of treatment). The indicated drug is used to induce cell death. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.


  • Quantification of % viable cells of Jurkat cells treated with a dose response of the inidicated drug (to induce cell death) and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry.


  • The sample dot plots demonstrate varying ratios of live and dead cells. More green = upper left = live cells; more red = lower right = dead cells.

  • Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with a drug to induce cell death were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.



This product has been referenced in:
  • Bahari L  et al. Directional freezing for the cryopreservation of adherent mammalian cells on a substrate. PLoS One 13:e0192265 (2018). Read more (PubMed: 29447224) »
  • Wang Q  et al. Evaluation of synergistic osteogenesis between icariin and BMP2 through a micro/meso hierarchical porous delivery system. Int J Nanomedicine 12:7721-7735 (2017). Read more (PubMed: 29089766) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

You can use same FITC and PE filters in this assay.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Unfortunately, we do not have free or trial sized samples of any of our products. We will guarantee this kit for differentiating between live and dead cells. If you are at all unsatisfied with the results, we are ...

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I have sent refund request to our accounts department on 13th June 2012. If you haven't received the refund please contact them by email mailto:creditcontrol@abcam.com or call 01223-696900 for accounts.

I ...

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Your credit note ID is XXXXXXX

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used ...

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Thank you for contacting us. I have been discussing this case with my colleagues, who developed this product; the following is their reply

-try staining the cells in PBS+dye (aspirate media, replace with 5X dye in PBS) [2b in microscopy secti...

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Thank you for your email. I am sorry to hear that you are experiencing problems with this kit.

I have checked the protocol and details you have kindly provided. It seems the dye is not entering the cells, which could be due to cells so is it ...

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Thank you for your enquiry. I am sorry to confirm we have not tested cell viability post-staining with this product and we would not be able to guarantee whether or notit would affect the cells you want to continue culturing. Ifyou would liketo test it...

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