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Live and Dead Cell Staining Kit (ab65470)

Submit a review Q&A (4)References (1)

Key features and details

  • Assay type: Direct
  • Detection method: Fluorescent
  • Platform: Microplate reader, Fluorescence microscope
  • Assay time: 20 min
  • Sample type: Adherent cells, Suspension cells
  • Sensitivity: 75 pg/ml

Overview

  • Product name

    Live and Dead Cell Staining Kit
    See all Live dead cell kits

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Direct

  • Sensitivity

    75 pg/ml

  • Range

    0.156 ng/ml - 10 ng/ml

  • Assay time

    0h 20m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mammals

  • Product overview

    This Live-Dead Cell Staining Kit provides the ready-to-use reagents for convenient discrimination between live and dead cells. The kit utilizes a cell-permeable green fluorescent dye (Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a cell non-permeable red fluorescent dye (Ex/Em = 536/615). Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine). The kit provides sufficient reagents for 100 stainings using 24-well plate.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Distinguishing between live and dead cells is very important for investigation of growth control and cell death.

  • Tested applications

    Suitable for: FMmore details

  • Platform

    Microplate reader, Fluorescence microscope

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab65470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM
Use at an assay dependent concentration.
Notes
FM
Use at an assay dependent concentration.

References (1)

Publishing research using ab65470? Please let us know so that we can cite the reference in this datasheet.

ab65470 has been referenced in 1 publication.

  • Saez I  et al. Neurons have an active glycogen metabolism that contributes to tolerance to hypoxia. J Cereb Blood Flow Metab N/A:N/A (2014). PubMed: 24569689

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

Customer kindly contacted to inquire as to what the Live/Dead Cell stain in this kit is, and to ask whether this may work with fresh, 70um, tissue slices.

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Answer

Thank you for contacting us.





I have been in touch with lab regarding this product. The only information I was able to obtain about the live/dead dye is that this is a cell-permeable green fluorescent dye (Ex/Em = 488/518 nm), which stains live cells.

Unfortunately, this kit can be used only with cells. It is not applicable to tissues.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

I am interested in your Live-Dead Cell Staining Kit (ab65470) I need an apoptosis assay for use on live cells. Specifically, I am looking at murine Megakaryocytes. We are treating them with different compounds to see which ones affect maturation and platelet production. We need to make sure, however, that these compounds aren't inducing apoptosis. Ideally, we would like to do this in a 96-well plate format to make it more high-throughput. We would treat the cells and monitor them over time (about 8-10 hours) and look at apoptosis in the same samples over time as well by immunofluorescence. We would like to do this by taking DIC and immunofluorescence images of each sample every hour for about 10 hours. I am wondering if your product would be appropriate for this application. Any advice or suggestions would be greatly appreciated. Thank you!

Read More
Answer

Thank you for contacting us.

I belleive that the Live-Dead Cell Staining kit that you have chosen will be an excellent choice, however this kit is optimized for 100 tests with 24-well plates. As an alternative, I would suggest ab115347 Live/Dead Cell Assay (https://www.abcam.com/Live-Dead-Cell-Assay-ab115347.html). This will provide you with enough materials to perform 1000 tests and would be better suited for the high throughput assays that you require.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Can this kit be used with cells that are still adhered to tissue culture plastic? I'm trying it, but seeing overlap of the red and green signals.

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Answer

Thank you for your inquiry. We have not specifically tested this, however with some optimizations it should work when the cells are cultured in the indicated manner as well. The recommendation from the protocol is "For analyzing adherent cells, grow cells directly on a coverslip. Following incubation with the Staining Solution, invert coverslip on a glass slide and visualize cells." Would you be able to do this instead? Also, as the optimal staining conditions may vary among different cell types, we recommend that a suitable concentration of Solution A and B be determined individually. As far as the overlap of signal, healthy cells stain only the cell-permeable Live-Dye, fluorescing green. Dead cells can stain both the cell-permeable Live-Dye and the cell non-permeable PI (red), the overlay of green and red appears to be yellow-red. I hope this information helps. Please let me know if you have any more questions. 

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Question

Can this kit be used on fixed cells?

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Answer

Thank you for your inquiry. Unfortunately, this kit is not compatible with fixed cells. Please do not hesitate to contact us if you need any more advice or information.

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