Overview

  • Product name

    Live Cell Labeling Kit - Blue Fluorescence - Cytopainter
    See all Cell labeling kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Assay time

    2h 00m
  • Product overview

    Live Cell Labeling Kit - Blue Fluorescence | Cytopainter (ab187963) is designed to uniformly label live cells in blue fluorescence with a proprietary dye whose fluorescence is strongly enhanced upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. Cells grown on black wall/clear bottom plates or slides can be stained and quantified in less than two hours. View cells at Ex/Em = 360/445 nm.


    This kit can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both suspension and adherent cells.

  • Notes

    Abcam’s CytoPainter Live Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations and flow cytometric investigations of cellular functions. The effective labelling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

Images

  • Image of CPA cells stained with CytoPainter Live Cell Labeling Kit - Blue Fluorescence (ab187963) in a black wall/clear bottom 96-well plate.

Protocols

References

ab187963 has not yet been referenced specifically in any publications.

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