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    live-cell-labeling-kit-green-fluorescence-ex405nm-cytopainter-ab187967.pdf

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Live Cell Labeling Kit - Green Fluorescence (Ex405nm) - Cytopainter (ab187967)

  • Datasheet
  • SDS
  • Protocol Booklet
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HeLa cells with CytoPainter Live Cell Labeling Kit - Green Fluorescence

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    Overview

    • Product name

      Live Cell Labeling Kit - Green Fluorescence (Ex405nm) - Cytopainter
      See all Cell labeling kits
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based
    • Product overview

      Live Cell Labeling Kit - Green Fluorescence (Ex405nm) | Cytopainter (ab187967) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time. The kit uses a non-fluorescent dye that carries a cell-retaining moiety. The dye becomes strongly fluorescent upon entering into live cells, and is trapped inside cells to give stable fluorescence signal that can be measured at Ex/Em = 410/500 when excited with a violet laser at Ex=405 nm. The dye is a hydrophobic compound that easily permeates intact live cells.


      The labeling process is robust, requiring minimal hands-on time. This product can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

    • Notes

      Abcam’s CytoPainter Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

    • Platform

      Flow cytometer, Fluorescence microscope

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 200 tests
      HHBS 1 x 100ml
      Labeling Dye Green (Ex405nm) 2 vials
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Cell Staining Kits
      • Cell Tracking kits

    Associated products

    • Related Products

      • Live Cell Labeling Kit - Blue Fluorescence - Cytopainter (ab187963)
      • Live Cell Labeling Kit - Green Fluorescence - Cytopainter (ab187964)
      • Live Cell Labeling Kit - Red Fluorescence - Cytopainter (ab187965)
      • Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) - Cytopainter (ab187966)

    Images

    • HeLa cells with CytoPainter Live Cell Labeling Kit - Green Fluorescence
      HeLa cells with CytoPainter Live Cell Labeling Kit - Green Fluorescence

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab187967? Please let us know so that we can cite the reference in this datasheet.

    ab187967 has been referenced in 1 publication.

    • Datta S  et al. Kidney Disease-Associated APOL1 Variants Have Dose-Dependent, Dominant Toxic Gain-of-Function. J Am Soc Nephrol 31:2083-2096 (2020). PubMed: 32675303

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