Overview

  • Product name

    Live Cell Labeling Kit - Red Fluorescence - Cytopainter
    See all Cell labeling kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Assay time

    2h 00m
  • Product overview

    Live Cell Labeling Kit - Red Fluorescence | Cytopainter (ab187965) is designed to uniformly label live cells in red fluorescence with a proprietary non-fluorescent dye that becomes strongly fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. Cells grown on slides or black wall/clear bottom plates can be stained and quantified in less than two hours.


    The assay is more robust than the tetrazolium salt one. It can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope with Ex/Em = 649/659 nm. It is also useful in a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. CytoPainter Live Cell Labeling Kit – Red Fluorescence (ab187965) provides all the essential components and can be used for both proliferating and non-proliferating cells.

  • Notes

    Abcam’s CytoPainter Live Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations and flow cytometric investigations of cellular functions. The effective labelling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

Images

  • Image of CPA cells stained with CytoPainter Live Cell Labeling Kit - Red Fluorescence (ab187965) in a black wall/clear bottom 96-well plate.

Protocols

References

ab187965 has not yet been referenced specifically in any publications.

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