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Recombinant full length Human liver FABP (aa1-127) purified from E. coli (NP_001434)
Our Abpromise guarantee covers the use of ab82157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/300. for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer.|
|WB||1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).|
|ELISA||Use at an assay dependent concentration.|
Ab82157 staining liver FABP in Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse IgG was used as the secondary antibody. DAPI was used as a nuclear counterstain.
ab82157 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"