Overview

  • Product name
    Anti-liver Arginase antibody
    See all Liver Arginase primary antibodies
  • Description
    Rabbit polyclonal to liver Arginase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ELISA, Conjugationmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Arginase [Bovine Liver]

  • General notes


    Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Primary antibody notes
    Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Use under non reducing condition. Detects a band of approximately 120 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Conjugation Use at an assay dependent dilution.

Target

  • Pathway
    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease
    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similarities
    Belongs to the arginase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

References

This product has been referenced in:
  • Nelin LD  et al. MKP-1 switches arginine metabolism from nitric oxide synthase to arginase following endotoxin challenge. Am J Physiol Cell Physiol 293:C632-40 (2007). Read more (PubMed: 17442735) »
  • Buchwalow IB  et al. Compartmentalization of NO signaling cascade in skeletal muscles. Biochem Biophys Res Commun 330:615-21 (2005). Read more (PubMed: 15796928) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your enquiry. This antibody has only been tested to react in cow, so we can not guarantee that this antibody will work with mouse samples. Typically, homology over 85% is a good measure of whether the antibody will work in that species or not. I do not know if you will get good results for mouse samples with this antibody. If you do purchase this antibody and use it in mouse, please fill out an Abreview and let us know how it went. By submitting an Abreview, you will earn Abpoints toward many promotions offered by Abcam. If you are using a cellular extract, you would want to add protease inhibitors with the lysis buffer in the same way that you would prepare whole cell samples for Western blot. You could use NP-40 or RIPA buffer for lysis. A list of protease inhibitors can be found by clicking the Resources tab on the Abcam homepage, then Protocols, Western Blot, and then Westen Blot: A beginners Guide. There is also more information on ELISA protocols on the Protocols page.

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Answer

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with human has not yet been tested for use with ab2111. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

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Answer

The antibody is raised against the whole native enzyme. It is reported Arginase II is expressed low in liver. We have not tested it with mouse or human and we are unable to predict the cross reactivity. The expected band size on SDS-PAGE is 120 kDa under non-reducing conditions.

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Answer

This polyclonal antibody reacts with arginase from most animal tissues. It quite possibly has cross-reactivity with human arginase. However, until it has been tested, we can't say for sure.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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