Overview

  • Product name

    Anti-liver Arginase antibody
    See all Liver Arginase primary antibodies
  • Description

    Goat polyclonal to liver Arginase
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Fr, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat Liver Arginase aa 311-323 (C terminal).
    Sequence:

    C-NHKPETDYLKPPK

  • Positive control

    • WB Rat liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).

observed in Mouse Liver and Rat Liver lysates

ICC 1/50.

Target

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similarities

    Belongs to the arginase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Images

  • All lanes : Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml

    Lane 1 : Mouse Liver lysates
    Lane 2 : Rat Liver lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 35 kDa



    Samples were incubated with prinary antibody for 1 hour. Detected by chemiluminescence.

  • Lane 1 : Anti-liver Arginase antibody (ab60176) at 0.05 µg/ml
    Lane 2 : Anti-liver Arginase antibody (ab60176)

    Lane 1 : Mouse Liver lysate in RIPA buffer
    Lane 2 : Rat Liver lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 35 kDa



    Detected by chemiluminescence. 

  • Anti-liver Arginase antibody (ab60176) at 1 µg/ml + Mouse liver tissue lysate in RIPA buffer at 35 µg

    Predicted band size: 35 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • ab60176 staining liver Arginase in murine liver tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and then blocked using 5% BSA for 30 minutes. Samples were then incubated with ab60176 at a 1/50 dilution for 1 hour. The secondary used was a biotinylated horse anti-goat IgG, used at a 1/200 dilution.

    See Abreview

  • Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml + Rat liver tissue lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • All lanes : Anti-liver Arginase antibody (ab60176) at 1/1000 dilution

    Lane 1 : Tissue extract prepared from mouse liver
    Lane 2 : Lysates prepared from RAW 264.7 murine macrophage cell line untreated
    Lane 3 : Lysates prepared from RAW 264.7 murine macrophage cell line treated with IL-4 10 ng/mL for 48 hours

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated rabbit anti-goat polyclonal IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds

    See Abreview

References

This product has been referenced in:

  • Wang G  et al. Age-Associated Dopaminergic Neuron Loss and Midbrain Glia Cell Phenotypic Polarization. Neuroscience 415:89-96 (2019). Read more (PubMed: 31325563) »
  • Kumaran Satyanarayanan S  et al. IFN-ß is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation. Nat Commun 10:3471 (2019). Read more (PubMed: 31375662) »
See all 16 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (Arm)
Permeabilization
No
Specification
Arm
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
Fixative
N/a

Miss. Crystal Jones

Verified customer

Submitted Nov 02 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Axolotl Tissue sections (Liver)
Antigen retrieval step
None
Permeabilization
No
Specification
Liver
Fixative
70% Ethanol

Abcam user community

Verified customer

Submitted Apr 27 2018

Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 22 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (dorsal root ganglion)
Antigen retrieval step
None
Permeabilization
No
Specification
dorsal root ganglion
Blocking step
Normal Donkey Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 18 2016

Application
Immunocytochemistry
Blocking step
Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Cell (Brain)
Specification
Brain
Permeabilization
Yes - 0.3-0.5% Triton X
Fixative
Paraformaldehyde

Dr. Joshua Brumberg

Verified customer

Submitted Jun 28 2013

Answer

Thank you for contacting Abcam and for your interest in ab60176 and ab3523. I have checked the online datasheets and both of these products have been validated in rat. Can you please confirm which application you will use them in? Once I have this information I can determine if the products are eligible for our testing discount. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Question
Answer

Sorry for the late reply. I am still enquirying as to the availability of these 2 antibodies in BSA-free and azide-free formulations. Thanks for your patience!

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 22 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW 264.7 murine macrophage cell line)
Loading amount
50 µg
Specification
RAW 264.7 murine macrophage cell line
Treatment
IL-4 10 ng.mL-1 for 48 hours
Gel Running Conditions
Reduced Denaturing (10% acrylamyde gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 22 2010

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