Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-liver Arginase antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 25% Normal Goat Serum
Dilution
1/1500

Secondary antibody

Name
Non-Abcam antibody was used: Vectastain Elite ABC Rabbit IgG Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Endogenous peroxidase quenched with 3% H2O2 in CH3OH for 10min

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Submitted Oct 14 2014

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