Immunohistochemistry (Frozen sections) abreview for Anti-liver Arginase antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Permeabilization
Yes - 0.2% Tween 20
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.2% Tween 20 + 1% BSA
Concentration
2 µg/ml

Secondary antibody

Dilution
1/500

Additional data

Additional Notes
Frozen sections were brought to room temperature and fixed with 4% PFA for 15 minutes. The sections were washed three times with TBS after each step of fixing, blocking/permeabilization, primary, and secondary incubation.

Stuart Young

Verified customer

Submitted Feb 28 2017

Sign up